甘草体外抑制呼吸道合胞病毒作用研究

目的:开发安全有效的抗呼吸道合胞病毒的新药.方法:采用细胞病变抑制实验观察甘草在Hela细胞中对呼吸道合胞病毒的抑制作用.结果:甘草半数中毒浓度(TC50)为3.43g/L;抑制呼吸道合胞病毒的半数有效浓度(EC50)为0.2535g/L,治疗指数(TI)为13.53;甘草对呼吸道合胞病毒的抑制作用存在量效反应关系;在感染后0 h、2 h、4 h、6 h、8 h,甘草均有抑制呼吸道合胞病毒的作用.结论:甘草在Hela细胞中对呼吸道合胞病毒有明显的抑制作用,其作用机制是多途径的.

The Anti-Respiratory Syncytial Virus (RSV) Effect of Radix Glycyrrhizae in Vitro

Objective:To develop safe and effective anti-RSV new medicine from Radix Glycyrrhizae.Method:The anti-RSV effect of Radix Glycyrrhizae in Hela cell culture was observed by means of the inhibition of cytopathic effect.Result:In Hela cell culture,Radix Glycyrrhizae was found to be a inhibitor of RSV in a concentration-dependent manner. The median toxic concentration(TC 50 ) of Radix Glycyrrhizae was 3.43 g/L,the median effective concentration (EC 50 ) of Radix glycyrrhizae against replication of the Long strain of RSV in Hela cells were 0.2535 g/L,the selectivity index (TI=TC 50 /EC 50 ) is 13.53.In time of addition experiment,Radix Glycyrrhizae inhibited the effect of RSV in Hela cells when it was added at 0h,2h,4h,6h,8h after virus infection.Conclusion:In Hela cell culture,Radix Glycyrrhizae was found to be a inhibitor of RSV,there are many ways in the mechanisms.