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A semiautomated method for the determination of 11-deoxy-17-oxo-steroids in urine |
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| Authors: | Ben Rademaker Anneke Jongejan-Timmer Jan Poortman Jos H.H. Thijssen |
| Affiliation: | Department of Endocrinology, Academic Hospital, State University of Utrecht, Catharijne singel 101, Utrecht, The Netherlands |
| Abstract: | A semiautomated method is described for the determination of total 11-deoxy-17-oxo-steroids (11-DOOS: androsterone, etiocholanolone plus dehydroepiandrosterone) in urine. Urinary conjugates are manually extracted on a XAD-2 resin, hydrolysed by (β-glucuronidase and “solvolysed” in acid ethyl-acetate according to Burnstein-Lieberman. The free 11-DOOS are extracted automatically with iso-octane and estimated colorimetrically by the Zimmerman reaction in an Auto-Analyzer II system. The method was evaluated by investigation of its precision, accuracy, sensitivity and specificity. It was found to be satisfactory for the rapid and reliable screening of large numbers of urine samples. |
| Keywords: | 11-DOOS 11-deoxy-17-oxo-steroids DHEA dehydroepian drosterone, 3β-hydroxy-5-androsten-17-one A androsterone, 3α-hydroxy-5α-androstan-17-onc E etiocholanolone, 3a-hydroxy-5β-androstan-17-one T testosterone, 17β-hydroxy-4-androsten-3- one G glucosiduronate S sulphate conjugate |
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