| 腺病毒载体介导肝细胞生长因子基因对血管内皮细胞的作用 |
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| 引用本文： | 刘本德,吴红军,熊汝芳,曾秋棠. 腺病毒载体介导肝细胞生长因子基因对血管内皮细胞的作用[J]. 中国康复, 2005, 20(6): 330-332 |
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| 作者姓名： | 刘本德 吴红军 熊汝芳 曾秋棠 |
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| 作者单位： | 1. 华中科技大学同济医学院,附属协和医院心血管研究所,湖北,武汉,430022
2. 华中科技大学同济医学院,生物化学与分子生物学系,湖北,武汉,430022 |
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| 基金项目： | 湖北省自然科学基金（No.2004ABA222） |
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| 摘 要： | 目的：研究腺病毒载体介导肝细胞生长因子（HGF）基因感染血管内皮细胞后在正常供氧、缺氧及缺氧后复氧的情况下细胞的凋亡情况。方法：将分离、培养的内皮细胞分为3组，分别给予M199（对照组）、HGF（HGF组）和HGF基因腺病毒载体（Ad-HGF组），分别在正常供氧、缺氧及缺氧后复氧3种情况下观察细胞的凋亡情况。结果：Ad-HGF组及HGF组细胞凋亡数均低于对照组（P〈0．01），Ad-HGF组与HGF组细胞凋亡数差异无显著性意义。结论：腺病毒载体介导HGF基因感染内皮细胞后能在缺氧情况下有效地阻止细胞凋亡。
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| 关 键 词： | 人重组腺病毒介导肝细胞生长因子基因 血管内皮细胞 凋亡 缺氧 |
| 文章编号： | 1001-2001（2005）06-0330-03 |
| 收稿时间： | 2005-02-22 |
| 修稿时间： | 2005-02-22 |
Effect of Adenovirus Vector Mediated Hepatocyte Growth Factor Gene on Vascular Endothelial Cells |
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| LIU Ben-de, WU Hong-jun, XIONG Ru-fang. Effect of Adenovirus Vector Mediated Hepatocyte Growth Factor Gene on Vascular Endothelial Cells[J]. Chinese Journal of Rehabilitation, 2005, 20(6): 330-332 |
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| Authors: | LIU Ben-de WU Hong-jun XIONG Ru-fang |
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| Affiliation: | Institute of Cardiovascular Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technologe, Wuhan 430022, China |
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| Abstract: | Objective:To investigate the apoptosis of vascular endothelial cells(VEC) transfected by adenovirus vector mediated hepatocyte growth factor gene(Ad-HGF) under condition of normoxia,anoxia and anoxia reoxygenation.Methods: The isolated and cultured VEC were divided into 3 groups and treated with M199(control group),HGF(HGF group) and Ad-HGF(Ad-HGF group) respectively.The apoptosis of the VEC was observed under different conditions of normoxia,anoxia and anoxia reoxygenation.Results:Under three conditions,the apoptotic cells in Ad-HGF group and HGF group were less than those in the control group(P<0.01).There was no significant difference in the apoptotic cells between Ad-HGF group and HGF group.Conclusion:HGF gene transfection into VEC mediated by adenovirus vector could effectively suppress apoptosis under anoxic condition. |
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| Keywords: | adenovirus vector mediated hepatocyte growth factor gene vascular endothelial cell apoptosis anoxia |
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