LPT技术在胸腹水肿瘤细胞诊断中的应用

目的 探讨液基薄层细胞学制片技术(Liquid-based preparation cytology test system, LPT)在胸腹水细胞学诊断中的应用价值.方法 60例胸腹水标本同时分别采用传统普通细胞涂片法以及液基细胞学方法 进行检查,将两组结果 进行比较,数据采用卡方检验.结果 LPT制片与传统涂片相比,前者细胞数量多、核染色质着色鲜明可靠、成团细胞中的单个细胞界限分明,染色背景清晰;传统涂片细胞核染色质着色均一、缺乏立体感,染色背景欠清晰.本组普通涂片确诊癌11例(19%),可疑癌12例(20%),阴性37例(61%);LPT制片确诊癌19例(31%),可疑癌5例(8%) ,阴性36例(60%) ,两种制片方法的恶性细胞确诊率差异有显著性(P＜0.05) .结论 LPT细胞学制片技术能够提高被检测胸腹水脱落细胞分布的集中程度,增强细胞核染色着色对比度,提高恶性细胞诊断率.

Application of Liquid-based preparation cytology test in the research of pleural effusion and the ascites

Aim To study the value of Liquid-based preparation cytology test system(LPT) in cytological diagnosis of pleural effusion and the ascites(PEA).Methods 60 cases of PEA were detected simultaneously and respectively using LPT and traditional cells smears.The consequence of the two groups were compared and analyzed with chi-square test.Results Comparing with the LPT and the trational cells smears,the former have the dominances of plentiful cells,vivid colouring of nuclear chromatin,distinct margin of single cell in mass cells,and clear background.Of these 60 cases,the carcinomatous cases diagnosed by the method of traditional cells smears were 11(19%),the doubtful carcinomatous cases were 12(20%),the negative cases were 37(61%).The carcinomatous cases diagnosed by the LPT were 19(31%),the doubtful carcinomatous cases were 5(8%),the negative cases were 36(60%).There was obvious difference in the diagnosis in malignant cells between them(P<0.05).Conclusion The LPT methods have dominance in improving the collective extent of caducaus PEA cells in making the sections,stronging the contrast of dying,and improving the rate of identifying the malignant cells.