补肾养骨汤诱导多发性骨髓瘤细胞株 KM3 的 凋亡及机制探讨

目的 探讨补肾养骨汤对人多发性骨髓瘤细胞株 KM3 细胞增殖、凋亡的影响及其机制。方法 取对数生 长期 KM3 细胞，分别加入 5%、10%、20% 的补肾养骨汤含药血清培养，培养 12、24、48 和 72 h 后，采用 Cell Counting Kit-8（CCK-8）法检测补肾养骨汤对 KM3 细胞增殖的影响；KM3 细胞经过不同浓度含药血清培养 72 h 后，采用流式 细胞术检测补肾养骨汤对 KM3 细胞周期和凋亡作用；RT-qPCR 和 Western blot 检测 B 淋巴细胞瘤-2 基因（Bcl-2）、 Bcl-2 相关蛋白（Bax）和核转录因子（NF）-κB 的表达水平。结果 补肾养骨汤抑制 KM3 细胞株的增殖，诱导 KM3 细胞株呈剂量依赖性凋亡，使 KM3 细胞周期停滞在 G0/G1 期，导致 KM3 细胞株 Bcl-2、NF-κB 表达下调，Bax 表达 上调。结论 补肾养骨汤抑制 KM3 细胞的增殖，影响细胞周期，诱导其凋亡，此作用机制可能与 Bcl-2、Bax 和 NF- κB 表达异常有关。

Inductive effect of BuShenYangGuTang on apoptosis of multiple myeloma cell line KM3 and its mechanism

Objective To study the effects and mechanisms of BuShenYangGuTang on proliferation and apoptosis of multiple myeloma cell line KM3. Methods The inhibitory effect of BuShenYangGuTang on cell proliferation was assessed by CCK-8. BuShenYangGuTang induced KM3 cell cycle arrest was analyzed by flow cytometry after propidium iodide staining. Flow cytometry was also used to analyze the cell apoptosis after Annexin V-FITC staining. The expressions of Bcl- 2, Bax and NF- κB were determined by RT-qPCR and Western blot assay. Results BuShenYangGuTang inhibited the KM3 cell proliferation in a dose-dependent manner. The expression levels of Bcl-2 and NF - κB were decreased, the expression level of Bax was increased, and the cell cycle was arrested in G0 / G1 phase after treatment with BuShenYangGuTang. Conclusion BuShenYangGuTang could inhibit the proliferation, arrest cell cycle and induce the apoptosis in KM3 cells, which may be related to the abnormal expressions of Bcl-2, Bax and NF-κB.