Cdc42在舌鳞状细胞癌中的表达及临床意义

目的： 探讨舌鳞癌组织、癌旁组织以及正常舌组织中细胞分裂周期蛋白42（cell division cycle 42，Cdc42）的表达及临床意义。方法： 选择青岛大学附属青岛市市立医院口腔颌面外科2014—2016年手术切除的舌鳞癌原发灶42例，采用免疫组织化学和免疫印迹法检测患者的组织标本（舌鳞癌、癌旁组织和正常舌组织）中Cdc42蛋白的表达水平，并分析其与临床病理参数的相关性。采用SPSS 13.0软件包对相应资料进行t检验与秩和检验。结果： Cdc42蛋白在舌鳞癌以及其癌旁组织中的阳性率分别为80.95%和52.38%，均高于正常舌组织（11.90%），2组间比较差异有统计学意义（P<0.05）。Cdc42蛋白的表达与舌鳞癌的病理分化程度无关（P>0.05），Cdc42蛋白定位在胞核的阳性分度与舌鳞癌的病理分化程度相关（P<0.05）；与患者的年龄、性别无关(P>0.05)；与颈淋巴结转移及舌鳞癌的临床分期呈正相关(P<0.05)。结论： Cdc42蛋白在舌鳞癌组织及癌旁组织中表达增高，与舌鳞癌的发生、发展密切相关。

Expression of Cdc42 in tongue squamous cell carcinoma and its clinical significance

PURPOSE: To investigate expression of cell division cycle 42 (Cdc42) and its clinical significance in tongue squamous cell carcinoma (TSCC). METHODS: Forty-two patients with TSCC were collected and the expression of Cdc42 in tumor, adjacent tissue and normal tissue was detected by immunohistochemistry and Western blot, respectively. The correlation of Cdc42 expression and clinical parameter were analyzed as well. Statistical analysis was performed using t test and rank sum test with SPSS 13.0 software package. RESULTS: The positive rate of Cdc42 in TSCC and adjacent tissue was 80.95% and 52.38%, respectively, which was higher than 11.90% in normal tissue. There was significant difference of Cdc42 expression between tumor, adjacent tissue and normal tissue (P<0.05). Cdc42 protein expression in cases with cervical lymph node metastasis was significantly higher than in cases without cervical lymph node metastasis (P<0.05). The expression of Cdc42 in TSCC had positive relation with clinical stages (P<0.05), but no correlation with pathological grade, age and gender (P>0.05). The expression levels of Cdc42 in nucleus were associated with pathological grade of TSCC. CONCLUSIONS: The results indicated Cdc42 positive expression is higher in TSCC and adjacent tissue. Over-expression of Cdc42 may play an important role in TSCC development and progress.