PARP-1对鼻咽癌CNE-2细胞迁移及侵袭能力的影响

目的 探讨聚腺苷二磷酸核糖聚合酶-1［poly（ADP-ribose）polymerase-1，PARP-1］对鼻咽癌CNE-2细胞迁移、侵袭能力的影响。方法 将鼻咽癌CNE-2细胞分为空白对照组（CNE-2组）、转染阴性病毒对照组（NC组）、转染稳定沉默PARP-1基因组（LV组）和未照射组（CNE-2-0 Gy组），4组细胞在照射前划痕、前3组给予10 Gy剂量照射，48 h后计算细胞迁移率、穿入下室细胞数，采用Western blot法检测PARP-1蛋白及Vimentin蛋白的表达。结果 照射后48 h，CNE-2组、NC组、LV组和CNE-2-0 Gy组的细胞迁移率分别为（79.37±5.75）%、（70.13±6.56）%、（20.83±10.91）%和（27.13±7.50）%；迁移穿入下室的细胞数分别为（60.00±2.66）个、（52.33±9.71）个、（13.67±3.51）个和（23.33±4.04）个，侵袭入下室细胞数分别为（33.00±2.00）个、（29.00±1.72）个、（6.67±2.52）个和（9.67±1.16）个；PARP-1蛋白相对表达量分别为1.00±0.00、1.02±0.04、0.47±0.05和0.47±0.06，Vimentin蛋白相对表达量分别为1.00±0.00、0.99±0.04、0.24±0.02及0.27±0.03；LV组细胞迁移率较CNE-2组及NC组降低，迁移及侵袭入下室的细胞数较少，PARP-1及Vimentin蛋白相对表达量下调，差异均有统计学意义（P<0.05）；LV组与CNE-2-0 Gy组比较，差异无统计学意义（P>0.05）。结论 PARP-1照射后激活可促进鼻咽癌CNE-2细胞迁移、侵袭。

Effect of PARP-1 on the invasion and migration of nasopharyngeal carcinoma cell line CNE-2

Objective To investigate the effect of ［poly（ADP-ribose）polymerase-1，PARP-1］ on migration and invasion by nasopharyngeal carcinoma cell line CNE-2. Methods The cells were divided into a blank control group （CNE-2 group），negative virus control group （NC group），silenced PARP-1 group （LV group） and non-irradiated group（CNE-2-0 Gy group）. Four groups of cultures were scratched and added to the transwell chamber before irradiation. The migration rate，the number of cells penetrating into the lower chamber，and the expression of PARP-1 and Vimentin were detected at 48 h after administration of 10 Gy X-ray irradiation，while the CNE-2-0 Gy group received 0 Gy. Results The migration rates of the CNE-2，NC，LV and CNE-2-0 Gy groups were，respectively，（79.37±5.75）%，（70.13±6.56）%，（20.83±10.91）% and（27.13±7.50）%. The numbers of cells in these groups that migrated into the lower chamber were 60.00±2.66，52.33±9.71，13.67±3.51 and 23.33±4.04. The number of invasive cells were 33.00±2.00，29.00±1.72， 6.67±2.52 and 9.67±1.16. The relative expression levels of PARP-1 were 1.00±0.00，1.02±0.04，0.47±0.05 and 0.47±0.06. The rela-tive expression levels of Vimentin were 1.00±0.00，0.99±0.04，0.24±0.02 and and 0.27±0.03 at 48 h after irradiation. The LV group showed significantly lower migration rate than the CNE-2 and NC groups，and it showed lower numbers of migrating and invasive cells，as well as lower relative expression of PARP-1 and Vimentin （P<0.05）. There was no significant difference between the LV and CNE-2-0 Gy groups. Conclusion The activation of PARP-1 after irradiation may promote migration and invasion by nasopharyngeal carcinoma CNE-2 cells.