IGF-1对6-OHDA诱导神经元氧化损伤的保护作用

目的 探讨胰岛素样生长因子-1（IGF-1）对6-羟基多巴胺（6-OHDA）诱导的PC12细胞氧化损伤的保护作用。方法 以25、50、100、150、200 μmol/L 的6-OHDA 处理PC12细胞，在12、24、48 h用MTT 法检测6-OHDA 对PC12细胞活性的影响，筛选最佳的实验浓度和观察时间。实验分为3组：对照组、6-OHDA组（150 μmol/L处理24 h）和IGF-1+6-OHDA组（IGF-1 100 nmol/L预处理2 h，后加入150 μmol/L 6-OHDA处理24 h），MTT法检测各组细胞活性；免疫荧光染色法检测细胞活性氧（ROS）水平；Hoechst33342/PI双染法检测细胞凋亡。结果 随6-OHDA浓度的增加和作用时间的延长，PC12细胞的活性呈梯度降低，150 μmol/L 6-OHDA浓度和处理后24 h作为本研究的最佳的实验浓度和观察时间。与6-OHDA组比较，IGF-1+6-OHDA组PC12细胞活力增强、ROS水平下降、细胞凋亡减少。结论 IGF-1预处理能减少6-OHDA引起的PC12细胞氧化损伤及凋亡，增加细胞活性，为防治帕金森病提供了潜在的治疗策略。

The protective effect of IGF-1 on 6-OHDA induced oxidative damage in neurons

Objective To investigate the protective effect of insulin-like growth factor-1 (IGF-1) on 6-hydroxydopamine (6-OHDA) induced oxidative damage in PC-12 cells. Methods PC12 cells were treated with 6-OHDA(concentrations of 25, 50, 100, 150 and 200 μmol/L). In order to select the optimal experimental concentration and treatment time, the activity of PC12 cells was detected by MTT at different time points of 12 h, 24 h and 48 h after treatment. PC12 cells were divided into three groups: control group, 6-OHDA group and IGF-1+6-OHDA group. The activity of PC12 cells was detected by MTT assay. Reactive oxygen species (ROS) level of PC12 cells was detected by immunofluorescence staining, and apoptosis was detected by Hoechst33342 / PI double staining method. Results With the increased concentration and the prolongation of the action time of 6-OHDA, the activity of PC12 cells decreased gradually. The concentration of 150 μmol/L and action time of 24 h of 6-OHDA were selected as the optimal experimental concentration and observation time for this study. Compared with 6-OHDA group, the activity of PC12 cells increased, the expression level of ROS and the apoptosis decreased in IGF-1+ 6-OHDA group. Conclusion IGF-1 pretreatment can reduce 6-OHDA induced oxidative damage and apoptosis of PC12 cells, also can increase cell activity, which can provide a potential strategy for the prevention and treatment of Parkinson’s disease.