泸州古蔺山银花指纹图谱及一测多评同时检测研究

目的:建立HPLC指纹图谱及一测多评同时检测泸州古蔺山银花中9个成分的质量分析方法。方法:以绿原酸为参照物,建立泸州古蔺山银花HPLC指纹图谱,并采用斜率校正法计算其与新绿原酸、隐绿原酸、獐牙菜苷、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸、灰毡毛忍冬皂苷乙及川续断皂苷乙的相对校正因子,分别用外标法和一测多评法计算含量。结果:建立了10批古蔺山银花HPLC指纹图谱,相似度均在0.99以上,标定了11个共有峰,指认了其中9个共有峰,并分别采用外标法和一测多评检测其含量,二者无显著差异。结论:实验将一测多评和指纹图谱相结合评价泸州古蔺山银花的质量,该方法准确、简便、可行,为更全面控制山银花的质量提供参考依据。

HPLC fingerprint and simultaneous determination of multi-components by single marker of Luzhou Guling Lonicerae Flos

OBJECTIVE To establish HPLC fingerprint and quantitative analysis of multi-components by single marker (QAMS) to simultaneous determine 9 composition in Luzhou Guling Lonicerae Flos.METHODS Chlorogcnic acid was selected as the marker of ingredients to establish the HPLC fingerprint of Guling Lonicerae Flos,and the relative correction factors of chlorogenic acid to neochlorogenic acid,cryptochlorogenic acid,sweroside,3,4-dicaffeoylquinic acid,3,5-dicaffeoylquinic acid,4,5-dicaffeoylquinic acid,macranthoidin B and dipsacoside B were calculated by slope correction method.The content of each component was determined by external standard method and QAMS method.RESULTS The HPLC fingerprints of 10 batches of Guling Lonicerae Flos were established.Eleven common peaks were identified with similarity of more than 0.99,among which 9 were verified and determined.There was no significant difference between the QAMS method and external standard method.CONCLUSION It is accurate and feasible to analyze the quality of Guling Lonicerae Flos by combining QAMS method and HPLC fingerprint,which can provide a reference support for the quality control of Lonicerae Flos.