基于正交试验优选益母草配方颗粒提取工艺

目的:运用正交试验优化益母草配方颗粒工艺提取,为益母草配方颗粒的生产工艺提供理论依据。方法:以料液比、提取次数和提取时间3个因素设计L9(34)正交试验,以水苏碱含量、益母草碱含量与浸膏得率三者的综合考察指标作为评价指标,优选益母草配方颗粒提取工艺。结果:优选工艺为料液比1∶12,提取2次,每次1.5 h,优化后的提取工艺经3个产地每个产地进3次平行验证试验,水苏碱的提取含量在34.29~35.93 mg·g^-1,益母草碱的提取含量在2.88~3.01 mg·g^-1,浸膏得率在25.11%~26.42%,3个评价指标的RSD均小于2.0%,以及三者的综合考察值在0.93~0.97。结论:优选的提取工艺方法稳定可靠,水苏碱、益母草碱及浸膏的提取率较高,为规范和标准化益母草配方颗粒生产研究工艺提供一定的参考依据。

Optimization of extraction process of motherwort formula granules based on orthogonal experiment

OBJECTIVE To optimize the extraction of motherwort granules by orthogonal experiment, and provide a theoretical basis for the producing process of motherwort granules.METHODS The comprehensive indexes of stachyose content, leonurine content and extract yields of three ingredients were used as evaluation indexes, L₉ (3⁴) orthogonal test was designed with three factors of material liquid ratio, extraction times and extraction time, to optimize the extraction process of motherwort formula granule.RESULTS The optimum process:solid-liquid ratio at 1:12, extracting for 2 times each for 1.5 h. The optimized extraction process was carried out in 3 parallel validation tests in each place of origin in 3 localities, the extraction content of stachydrine was 34.29~35.93 mg·g^-1, content of leonurine 2.88~3.01 mg·g^-1, the rate of extract yield 25.11%~26.42%, 3 evaluation indexes of RSD were less than 2%. The comprehensive investigation value of the three was 0.93~0.97.CONCLUSION The optimized extraction process is stable and reliable, and the extraction rates of stachydrine, leonurine and extract are higher, which provides a certain reference for regulating and standardizing the production process of motherwort formula granules.