Graves病合并牙周炎患者IL-6和TNF-α检测及意义

目的: 通过检测Graves病（GD）伴牙周炎(CP)患者龈沟液及血清中白介素6(IL-6)和肿瘤坏死因子α(TNF-α)的浓度,探讨Graves病与牙周炎之间的关系。方法: 采用双免疫酶联法检测30例健康志愿者、30例牙周炎患者、30例Graves病患者、30例Graves病伴牙周炎患者龈沟液及血清中IL-6和TNF-α含量。记录4组研究对象的牙周探诊深度（PD）、临床附着丧失(CAL)和龈沟出血指数（SBI）。采用SPSS 19.0软件包对数据进行统计学处理。结果: GD伴牙周炎组患者血清和龈沟液中IL-6和TNF-α的浓度显著高于单纯牙周炎组及GD组（P<0.05）,GD伴牙周炎组和GD组血清和龈沟液IL-6、TNF-α水平升高与FT3、FT4呈正相关（P<0.05）,且GD伴牙周炎组的相关性显著高于GD组。结论: Graves伴牙周炎组IL-6与TNF-α浓度高于单纯牙周炎组和Graves病组,表明在免疫机制方面,牙周炎与Graves病可能存在相互影响。

Detection and significance of IL-6 and TNF-α in patients with Graves disease and periodontitis

PURPOSE: To investigate the expression of IL-6 and TNF-α in gingival crevicular fluid (GCF) and serum of patients with Graves' disease(GD) and periodontitis (CP), and to analyze the correlation between Graves' disease and periodontitis. METHODS: One hundred and twenty subjects were selected and divided into 4 groups: 30 healthy participants as the control group, 30 patients with CP, 30 patients with GD, and 30 patients with GD and CP. The serum and GCF level of IL-6 and TNF-α were measured by enzyme-linked immunosorbent assay (ELISA), and clinical periodontal parameters including probing depth (PD), clinical attachment level (CAL) and sulcus bleeding indexes (SBI) were examined. The date was analyzed with SPSS19.0 software package. RESULTS: The concentration of IL-6 and TNF-α in serum and GCF of patients with GD+CP group was significantly higher than that in CP group and GD group (P<0.05). The level of IL-6 and TNF-α in serum and GCF of patients in GD+CP group and GD group was positively correlated with FT3 and FT4 (P<0.05), and the correlation between GD+CP group was significantly higher than that in GD group. CONCLUSIONS: The level of IL-6 and TNF-α in GD+CP group were significantly higher than those in CP group and GD group, indicating that there is an interaction between periodontitis and Graves' disease in immune mechanism.