苯扎溴铵和西酞普兰对小鼠角膜上皮和角膜全层厚度的影响

目的　通过对光学相干断层扫描血管造影技术（optical coherence tomography angiography，OCTA）的应用，探究苯扎溴铵和西酞普兰对小鼠角膜上皮和角膜全层厚度的影响。方法　选取60只小鼠随机均分为5组，其中A组为阴性对照组，B组用PBS眼液滴眼；C组用苯扎溴铵眼液滴眼；D组腹腔注射西酞普兰混悬液；E组苯扎溴铵眼液滴眼联合腹腔注射西酞普兰混悬液。在注射（滴眼）后2周，利用OCTA进行角膜分区，分别测量5组小鼠角膜各区域的角膜上皮和角膜全层厚度，并计算平均值。结果　A组角膜上皮及角膜全层厚度分别为（66±7）μm、（141±11）μm，B、D两组角膜上皮均为（66±8）μm，角膜全层厚度均为（140±12）μm，C组分别为（73±10）μm、（141±14）μm，E组分别为（76±12）μm、（141±15）μm。注射（滴眼）后2周，与注射（滴眼）前相比，A组、B组及D组角膜上皮厚度及角膜全层厚度略有变化，差异均无统计学意义（均为P＞0.05），而C组和E组各区域角膜上皮厚度及角膜全层厚度明显增厚，差异均有统计学意义（均为P＜0.05）。注射（滴眼）后2周，E组较C组相比，角膜上皮厚度及角膜全层厚度增厚更为显著，差异有统计学意义（P＜0.05）。各组注射后与注射前相比，C组和E组角膜上皮厚度和角膜全层厚度平均值明显增大，差异有统计学意义（均为P＜0.05）；与A组相比，C组和E组角膜上皮厚度和角膜全层厚度平均值明显增大，差异均有统计学意义（均为P＜0.05）。结论　应用OCTA测量发现，单独使用西酞普兰对角膜厚度无明显影响，苯扎溴铵可使角膜上皮及角膜全层厚度增厚，且西酞普兰对其增厚具有协同作用。

Effects of benzalkonium bromide and citalopram on the thickness of corneal epithelium and full-thickness of the cornea in mice

Objective　To explore the effects of benzalkonium bromide and citalopram on the corneal epithelium and corneal thickness of mice using optical coherence tomography angiography (OCTA).Methods　Together 60 mice were randomly divided into 5 groups (group A，B，C，D and E；n=12)，with group A left untreated，group B receiving PBS eye drops，group C given benzalkonium bromide eye drops，group D undergoing intraperitoneal administration of citalopram suspension，and group E treated with combination of benzalkonium bromide eye drops and citalopram suspension.After 2 weeks，OCTA was applied for corneal subarea，followed by measurement of the thickness of corneal epithelium and full-thickness of the cornea of all mice，and then the mean values were calculated.Results　The thickness of corneal epithelium and full-thickness of the cornea was (66±7)μm and (141±11)μm in the group A，(66±8)μm and (140±12)μm in the group B and D，(73±10)μm and (141±14)μm in the group C，(76±12)μm and (141±15)μm in the E group，respectively.And there was no significant difference in the thickness of corneal epithelium and full-thickness of the cornea before treatment and 2 weeks after treatment in the group A，B and D (all P>0.05)，but both variables were markedly thickened in group C and E 2 weeks after treatment，and the difference was statistically significant (all P<0.05).Moreover，the increased levels on the both variables in the group E was higher than those in the group C 2 weeks after treatment，and the difference was statistically significant (both P<0.05).The average thickness of corneal epithelium and full-thickness of the cornea in the group C and E were significantly thickened after treatment，and the difference was statistically significant (all P<0.05).The average values of both variables in the group C and E were obviously larger than those in the group A，and the difference was statistically significant (all P<0.05).Conclusion　Citalopram alone has no significant effects on the corneal thickness by OCTA，whereas both the thickness of corneal epithelium and full-thickness of the cornea tend to thicken by benzalkonium bromide treatment，which has a synergistic effect on corneal thickening with citalopram.