FOXK1对肝癌高转移细胞系Huh7侵袭和迁移能力的影响

目的 探讨叉头框转录因子1(FOXK1)对肝癌高转移细胞Huh7侵袭和迁移能力的影响。方法 采用实时定量逆转录聚合酶链式反应(qRT-PCR)检测40例肝癌组织及其癌旁正常组织FOXK1 mRNA的表达,qRT-PCR和Western blot检测正常肝细胞HL7702、肝癌细胞BEL7402和肝癌高转移细胞Huh7中FOXK1 mRNA和蛋白的表达。小RNA干扰技术下调FOXK1表达后,qRT-PCR和Western blot检测肝癌高转移细胞Huh7 FOXK1 mRNA和蛋白的表达,Transwell实验检测其侵袭和迁移能力。 结果 qRT-PCR检测结果显示,肝癌组织中FOXK1 mRNA的表达高于癌旁正常组织(2.87±0.21 vs 1.35±0.11,P=0.004);qRT-PCR和Western blot检测结果显示,相比正常肝细胞HL7702,肝癌细胞BEL7402和肝癌高转移细胞Huh7中FOXK1 mRNA和蛋白表达升高(P<0.001)。下调FOXK1表达后,肝癌高转移细胞Huh7中FOXK1 mRNA和蛋白表达显著下调(P<0.01),侵袭和迁移细胞数目明显减少(P<0.01)。结论 下调FOXK1表达能抑制肝癌高转移细胞Huh7侵袭和迁移能力。

Effect of FOXK1 on the invasion and migration of hepatocellular carcinoma cell line Huh7

Objective To investigate the influence of FOXK1 on the invasion and migration of hepatocellular carcinoma cell line Huh7. Methods Levels of  FOXK1 were examined in 40 cases of liver cancer and adjacent normal tissues using real-time PCR(qRT-PCR). The expression of FOXK1 in normal hepatocellular cells HL7702, hepatocellular carcinoma cells BEL7402 and hepa-tocellular carcinoma cell line Huh7 were measured using qRT-PCR and Western blotting, and influence of FOXK1 on invasion and migration of hepatocellular carcinoma cell line was detected by transwell assay. Results Levels of FOXK1 mRNA were significantly higher in  liver cancer tissues than in adjacent normal tissues(2.87±0.21 vs 1.35 ±0.11, P=0.004). Similarly,levels of FOXK1 mRNA and protein were significantly lower in normal hepatocellular cells HL7702 than in hepatocellular carcinoma cells BEL7402 or hepatocellular carcinoma cell line Huh7(P<0.001). After down-regulated the expression of FOXK1, levels of FOXK1 mRNA and protein were significantly down-regulated in hepatocellular carcinoma cell line Huh7(P<0.01), with lower numbers of invasive and migrating cells(P<0.01). Conclusions Down regulate the expression of FOXK1 can effectively suppress cell migration and invasion in hepatocellular carcinoma cell line Huh7.