| PRL-3对结直肠癌细胞迁移、侵袭能力的影响 |
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| 引用本文： | 董文刚1,' target='_blank'>2,刘 军2,' target='_blank'>3,褚 楚2,' target='_blank'>3,穆 楠2,' target='_blank'>3,顾锦涛2,' target='_blank'>3,张旺倩2,' target='_blank'>3,薛晓畅2,' target='_blank'>3,张英起2,' target='_blank'>3,张 伟2,' target='_blank'>3,张 勇1,钱济先1. PRL-3对结直肠癌细胞迁移、侵袭能力的影响[J]. 现代肿瘤医学, 2018, 0(12): 1815-1818. DOI: 10.3969/j.issn.1672-4992.2018.12.002 |
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| 作者姓名： | 董文刚1 ' target='_blank'>2 刘 军2 ' target='_blank'>3 褚 楚2 ' target='_blank'>3 穆 楠2 ' target='_blank'>3 顾锦涛2 ' target='_blank'>3 张旺倩2 ' target='_blank'>3 薛晓畅2 ' target='_blank'>3 张英起2 ' target='_blank'>3 张 伟2 ' target='_blank'>3 张 勇1 钱济先1 |
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| 作者单位： | 1.中国人民解放军空军军医大学唐都医院骨科，陕西 西安 710032；2.中国人民解放军空军军医大学药学系生物制药学教研室；3.肿瘤生物学国家重点实验室，陕西 西安 710032 |
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| 基金项目： | National Natural Science Foundation of China（No.81672654）；国家自然科学基金面上项目（编号:81672654） |
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| 摘 要： | 目的:构建肝再生磷酸酶-3（phosphatase of regenerating liver-3,PRL-3）基因过表达的重组慢病毒载体，研究其对结直肠癌细胞系HCT116迁移、侵袭能力的影响。方法:PCR扩增PRL-3基因并克隆至pcDH载体，构建成pcDH-PRL-3过表达载体，再将其与慢病毒包装载体共转染293T细胞，包装成Lenti-PRL-3重组慢病毒载体，并感染结直肠癌细胞系HCT116。利用qPCR、Western分别从mRNA、蛋白水平检测PRL-3表达情况；通过划痕实验、Transwell实验研究过表达PRL-3对结直肠癌细胞系HCT116侵袭能力的影响。结果:成功构建Lenti-PRL-3重组慢病毒载体，并在结直肠癌细胞系HCT116过表达。划痕实验、Transwell实验结果表明过表达PRL-3能够促进HCT116细胞的迁移、侵袭。结论:构建Lenti-PRL-3重组慢病毒载体，在结直肠癌细胞系HCT116过表达PRL-3后能增加细胞的迁移、侵袭能力。
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| 关 键 词： | PRL-3 慢病毒载体 结直肠癌 细胞侵袭 细胞迁移 |
The effects of PRL-3 on the migration and invasion of colorectal cancer cells |
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| Dong Wengang1,' target='_blank'>2,Liu Jun2,' target='_blank'>3,Chu Chu2,' target='_blank'>3,Mu Nan2,' target='_blank'>3,Gu Jintao2,' target='_blank'>3,Zhang Wangqian2,' target='_blank'>3,Xue Xiaochang2,' target='_blank'>3,Zhang Yingqi2,' target='_blank'>3,Zhang Wei2,' target='_blank'>3,Zhang Yong1,Qian Jixian1. The effects of PRL-3 on the migration and invasion of colorectal cancer cells[J]. Journal of Modern Oncology, 2018, 0(12): 1815-1818. DOI: 10.3969/j.issn.1672-4992.2018.12.002 |
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| Authors: | Dong Wengang1 ' target='_blank'>2 Liu Jun2 ' target='_blank'>3 Chu Chu2 ' target='_blank'>3 Mu Nan2 ' target='_blank'>3 Gu Jintao2 ' target='_blank'>3 Zhang Wangqian2 ' target='_blank'>3 Xue Xiaochang2 ' target='_blank'>3 Zhang Yingqi2 ' target='_blank'>3 Zhang Wei2 ' target='_blank'>3 Zhang Yong1 Qian Jixian1 |
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| Affiliation: | 1.Department of Bone，Tangdu Hospital，Air Force Medical University，Shaanxi Xi'an 710032,China；2.Department of Biopharmaceutics，Pharmacy Department；3.State Key Laboratory of Cancer Biology,Air Fourth Medical University，Shaanxi Xi'an 710032,China. |
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| Abstract: | Objective:To construct and identify combinant lentiviral vector for overexpression of PRL-3,and to study the effect on the invasion of the colorectal cancer cell HCT116.Methods:PRL-3 gene was amplified by using PCR and cloned into the pCDH vector.The resulting plasmid pCDH-PRL-3 with the packing carrier were used to transfect HCT116 cells.Then，examine the expression level of PRL-3 in HCT116 cells by performing the Western blot and qPCR，wound scratch assay and Transwell were used to study the effects of PRL-3 on the invasion of the HCT116 cells.Results:The recombinant lentivirus overexpressed PRL-3 in HCT116 cells.Wound scratch assay and Transwell indicated that overexpression of PRL-3 can promote the invasion of HCT116 cells.Conclusion:Recombinant lentivirues overexpressing PRL-3 were successfully constructed.Upregulation of PRL-3 can promote the invasion of HCT116 cells. |
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| Keywords: | PRL-3 ientivirus vector colorectal cancer cell invasion cell migration |
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