基因沉默策略及其应用研究进展

基因沉默 (Gene silencing) 是生物体中特定基因由于各种原因不表达或表达减少的现象, 是通过表观遗传控制基因表达的重要机制, 通过基因沉默技术探索疑难疾病的治疗方法是当下的研究热点.随着基因工程的迅速发展, 基因沉默技术不断有新技术出现取代已有的技术.对基因沉默技术所包括的核酶技术、反义寡核酸技术、基因敲除、RNA干扰技术及其应用进行介绍.

Advances and Applications in Gene Silencing

Gene silencing is a phenomenon in which the specific genes in organisms are not expressed or reduced for various reasons. It is an important mechanism to control gene expression by epigenetic control. At present, scientists intend to explore the treatment of difficult diseases through gene silencing technology, and the research has become a hot spot. With the rapid development of gene engineering, gene silencing technology has been replaced by new technologies. This paper aims to introduce the ribozyme technology, antisense oligonucleic acid technology, gene knockout, RNA interference technology and their applications in gene silencing technology.