HIP1在食管癌中的表达及其临床意义

目的:探究亨廷顿蛋白相互作用蛋白1(Huntingtin interacting protein 1,HIP1)基因和蛋白在食管癌组织中的表达及其临床意义。方法:采用免疫组织化学法检测85例食管癌组织及其对应癌旁组织中HIP1蛋白的表达，并分析该表达与食管癌患者临床病理特征的关系。荧光定量PCR及Western blot法检测HIP1基因和蛋白在食管癌及其癌旁组织中的表达。结果:HIP1在食管癌组织中的阳性表达率为91.8%（78/85），显著高于食管癌旁组织（5.9%，5/85），差异具有统计学意义（P<0.001）。统计分析发现，HIP1在病理分级组间差异显著（P=0.03），而在性别、年龄、TNM分期组间无统计学差异（P>0.05）。荧光定量PCR及Western blot结果发现，与食管癌旁组织相比，HIP1基因和蛋白在食管癌组织中高表达。结论:HIP1蛋白可能是食管癌组织和癌旁组织中的差异蛋白，可能是食管癌新的分子标志物。

Expression of HIP1 in esophageal squamous cell carcinoma and its clinical significance

Objective:To explore the gene and protein expression of HIP1 and its clinical significance in esophageal squamous cell carcinoma (ESCC).Methods:HIP1 expression was assessed using immunohistochemical (IHC) in 85 ESCC and adjacent ESCC tissues and the association of HIP1 expression with clinicopathological parameters were also evaluated.qRT-PCR and Western blot were used to detect the gene and protein expression of HIP1 in ESCC and adjacent ESCC tissues.Results:The expression rate of HIP1 in ESCC (91.8%，78/85) showed extremely significance compared with adjacent ESCC tissues (5.9%，5/85) (P<0.001).HIP1 expression was significantly associated with pathological grading (P=0.03)，but there were no significant differences with gender，age，TNM stage (P>0.05).qRT-PCR and Western blot results showed that compared with adjacent ESCC tissues，HIP1 gene and protein were higher expressed in ESCC tissues.Conclusion:HIP1 is a differential protein in cancer tissue and adjacent ESCC tissues and can serve as a new molecular biomarker in ESCC.