5-Aza-CdR对人脑胶质瘤细胞miR-129-2表达水平及其生物学行为的影响

目的:探讨DNA甲基转移酶抑制剂5-氮杂-2'-脱氧胞苷（5-Aza-CdR）对人脑胶质瘤细胞U87和U373中miR-129-2表达水平及细胞增殖、侵袭迁移能力的影响。方法:以0.5 mmol/L的5-Aza-CdR处理人脑胶质瘤细胞U87和U373 72 h，采用RT-PCR法检测miR-129-2的表达水平，CCK-8法观察胶质瘤细胞增殖能力，Transwell试验检测细胞的侵袭能力，划痕实验检测细胞的迁移能力。结果:经5-Aza-CdR处理后人脑胶质瘤细胞U87和U373的miR-129-2表达水平显著上调，细胞增殖能力、侵袭迁移能力受到抑制。结论:5-Aza-CdR能使miR-129-2启动子去甲基化，使其表达上调，并能抑制胶质瘤细胞增殖、侵袭迁移能力。

Effects of 5-Aza-CdR on miR-129-2 gene expression and biological behaviors of human glioma cell line

Objective:To study the effects of methyltransferase inhibitor 5-Aza-2'-deoxycytidine（5-Aza-CdR） on the miR-129-2 expression and cell proliferation，invasion，migration and cell cycle in human glioma cell line U87 and U373.Methods:U87 and U373 were treated and cultured with 0.5 mmol/L 5-Aza-CdR.RT-PCR was used to detect miR-129-2 expression level.CCK-8，transwell and gap test were used to detect cell proliferation，invasion and migration ability of U87 and U373 cell lines.Results:After treated with 0.5 mmol/L 5-Aza-CdR，miR-129-2 expression level was up-regulated remarkably and cell proliferation,invasion and migration ability were inhibited.Conclusion:5-Aza-CdR can reverse the methylation status of miR-129-2 and restore miR-129-2 expression.It can effectively inhibit proliferation，invasion and migration of human glioma cells.