喉鳞状细胞癌中SIAH2表达的预后价值

  目的  观察E3泛素连接酶SIAH2在喉鳞状细胞癌组织中的表达, 探讨其在喉鳞状细胞癌中表达的预后价值。  方法  应用免疫组织化学染色法检测119例喉组织标本SIAH2的定性表达, Western blot方法检测喉新鲜组织中SIAH2的定量表达, 结合患者5年随访资料, 利用Kaplan-Meier法绘制生存曲线, Log-rank法分析SIAH2的表达水平与喉鳞状细胞癌患者预后的关系, Cox比例风险回归模型分析喉鳞状细胞癌患者预后的独立预测因素。  结果  SIAH2在喉鳞状细胞癌中阳性表达率为77.19%, 显著高于不典型增生(53.13%)和癌旁正常喉组织(26.67%), 差异具有统计学意义(χ2=21.02, P＜0.001)。SIAH2的表达与组织学分级、临床分期及淋巴结转移相关(均P＜0.05);在正常喉组织(1.25±0.04)、不典型增生(1.38±0.05)及喉鳞状细胞癌组织(1.44±0.07)中SIAH2的相对表达量逐渐升高(F=61.811, P＜0.001);SIAH2阳性与阴性患者的5年生存率分别为18.18%和58.33%(χ2=5.720, P= 0.017), SIAH2阳性与阴性患者的中位生存时间分别为25个月和60个月(P＜0.05);多因素回归分析表明高表达SIAH2是喉鳞状细胞癌患者总生存期的独立预测因子。  结论  SIAH2可能作为一个癌基因参与喉鳞状细胞癌的发生发展, 过表达SIAH2与喉鳞状细胞癌患者的不良预后有关, 提示SIAH2作为一个潜在的靶基因可能对喉鳞状细胞癌的治疗有帮助。 

Prognostic value of SIAH2 expression in laryngeal squamous cell carcinoma

  Objective  :To investigate the prognostic value of SIAH2 expression in laryngeal squamous cell carcinoma (LSCC).  Methods  The qualitative expression of SIAH2 in 119 laryngeal tissues was studied by immunohistochemical staining.Western blot was used to examine the quantitative expression of SIAH2.Survival rates were calculated using the Kaplan-Meier method.Correlation between SIAH2 expression and LSCC patients'prognosis was analyzed by the Log-rank test.The Cox proportional hazards regression model was used to examine the independent predictive factors of LSCC.  Results  The SIAH2 expression in LSCC (77.19%) was higher than that in the laryngeal atypical hyperplasia (53.13%) and normal laryngeal tissues (26.67%), and significant differences were observed (χ2=21.02, P=0.000).The expression of SIAH2 was significantly correlated to the histological grade, clinical stage, and lymph node metastasis (P < 0.05).The relative expression of SIAH2 in normal laryngeal tissue (1.25±0.04), laryngeal atypical hyperplasia (1.38 ± 0.05), and LSCC (1.44±0.07) was observed to increase gradually (F=61.811, P < 0.001).The 5-year survival rate of SIAH2(+) and SIAH2(-) patients was 18.18% and 58.33%, respectively (χ2=5.720, P=0.017), and the median survival time of SIAH2(+) and SIAH2(-) patients was 25 and 60 months, respectively (P < 0.05).The multivariate regression analysis revealed that the higher expression of SIAH2 was an independent prognostic factor for the overall survival.  Conclusions  SIAH2 may be involved in the tumorigenesis and progression of LSCC as an oncogene.Overexpression of the marker indicated poor prognosis of the disease, a finding which might allow SIAH2 to be used as a potential target gene for the treatment of LSCC.