三氧化二砷对肝癌HepG2细胞侵袭转移及USP22蛋白表达的影响

目的:探究不同浓度三氧化二砷(As2O3)对肝癌HepG2细胞侵袭转移能力的影响以及对HepG2细胞中USP22蛋白表达的影响，进一步完善其分子机制。方法:CCK8细胞增殖实验检测不同浓度As2O3在不同时间点对HepG2细胞增殖的影响；细胞划痕和Transwell方法检测HepG2细胞在不同浓度As2O3作用下侵袭转移能力的变化；Western blot检测As2O3作用下HepG2细胞中USP22蛋白的表达水平。结果:随着As2O3浓度和作用时间的增加，HepG2细胞增殖明显受到抑制。与对照组相比，24 h时分别用2、4、8 μmol/L的As2O3处理的HepG2细胞侵袭转移能力降低，细胞中USP22蛋白表达水平明显下降。结论:As2O3可抑制人肝癌HepG2细胞侵袭转移能力，其分子机制可能与下调的USP22蛋白水平相关。

Effects of arsenic trioxide on invasion and migration and USP22 expression in human HepG2 hepatocarcinoma cells

Objective:To investigate the effects of arsenic trioxide (As2O3) of various concentrations on invasion，migration and USP22 expression in human HepG2 hepatocarcinoma cells and the underlying molecular mechanism.Methods:CCK8 was used to test the proliferation levels of HepG2 cells treated with As2O3 of different concentrations in different time.Wound-healing and Transwell assays were used to examine the effects of As2O3 of different concentrations on cell migration and invasion.The protein levels of USP22 treated with As2O3 of different concentrations were determined by Western blot.Results:With the increase of As2O3 concentration and time，the proliferation of HepG2 cells was obviously inhibited.The migration，invasion and the expression of USP22 protein in HepG2 cells which were treated with 2，4，8 μmol/L As2O3 respectively for 24 h were obviously decreased compared with the control group.Conclusion:As2O3 could inhibit the migration and invasion of human hepatocellular carcinoma HepG2 cells，which may be related to the down-regulation of USP22 protein．