PTEN基因对急性淋巴细胞白血病细胞增殖、侵袭的影响

目的:观察第10号染色体同源缺失性磷酸酶-张力蛋白同源的基因（PTEN）对急性淋巴细胞白血病细胞增殖、侵袭的影响。方法:在人急性淋巴细胞白血病细胞CEM-C1中转染PTEN过表达载体（pBP-PTEN），同时转染对照载体（pBP），以没有转染的细胞作为对照，RT-PCR和Western blot检测转染后细胞中PTEN的水平；MTT和集落形成试验检测细胞增殖能力；Transwell小室检测细胞侵袭和迁移能力；Western blot检测PTEN、基质金属蛋白酶-2（MMP-2）、基质金属蛋白酶-9（MMP-9）、p38丝裂原活化蛋白激酶（p38MAPK）、磷酸化的p38MAPK（p-p38MAPK）的水平。结果:转染对照载体的CEM-C1细胞中PTEN水平、细胞光密度（OD）值、克隆形成数目、侵袭数目、迁移数目及PTEN、MMP-2、MMP-9、p38MAPK、p-p38MAPK水平与未转染细胞相比没有明显变化（P＞0.05）。转染PTEN过表达载体后的CEM-C1细胞中PTEN水平和p-p38MAPK水平升高，细胞OD值、克隆形成数目、侵袭数目、迁移数目和MMP-2、MMP-9水平降低，与未转染细胞相比差异具有统计学意义（P＜0.05）。结论:PTEN抑制急性淋巴细胞白血病细胞增殖、侵袭及迁移，促进p38MAPK信号通路激活。

Effect of PTEN on the proliferation and invasion of acute lymphoblastic leukemia

Objective:To investigate the effect of PTEN on the proliferation and invasion of acute lymphoblastic leukemia cells.Methods:In human acute lymphoblastic leukemia cells,CEM-C1 was transfected with PTEN overexpression vector（pBP-PTEN) and transfected into the control vector（pBP),the cells without transfection were used as controls.The levels of PTEN in transfected cells were detected by RT-PCR and Western blot,MTT and colony forming assays were used to detect cell proliferation,the cell invasion and migration ability was detected by Transwell.PTEN,MMP-2,MMP-9,p38MAPK,p-p38MAPK protein levels were detected by Western blot.Results:PTEN levels,OD value,colony forming number,invasion number,migration number and PTEN,MMP-2,MMP-9,p38MAPK,p-p38MAPK levels in CEM-C1 cells transfected with the control vector had no obvious changes compared with the non transfected cells（P＞0.05).After the transfection of PTEN increased expression of PTEN and p-p38MAPK level vector in CEM-C1 cells,cell clone formation,invasion number,OD value,migration number and MMP-2,MMP-9 level reduced compared with non transfected cells,the difference was statistically significant（P＜0.05).Conclusion:PTEN inhibits the proliferation,invasion and amigration of acute lymphoblastic leukemia and promotes the activation of p38MAPK signaling pathway.