DAPK蛋白、Ki-67蛋白在子宫内膜癌中的表达及其与临床病理学特征的相关性

目的:探讨DAPK蛋白、Ki-67蛋白在子宫内膜癌中的表达及其与临床病理学特征的相关性。方法:选取2012年6月至2016年10月在我院及邯郸市第一医院治疗的子宫内膜癌患者150例，同时选取150例子宫内膜增生患者，采用免疫组化法检测DAPK蛋白、Ki-67蛋白表达情况，采用全自动生化法检测血清中CEA、CA19-9和CA125等的表达，分析 DAPK蛋白、Ki-67蛋白的表达与子宫内膜癌临床病理特征的关系。结果:子宫内膜癌患者组织中的DAPK蛋白低于子宫内膜增生组（P＜0.05），Ki-67蛋白阳性表达率均高于子宫内膜增生组患者（P＜0.05）；子宫内膜癌组患者CEA、CA19-9和CA125水平均高于子宫内膜增生组（P＜0.05）；子宫内膜癌患者的DAPK阳性表达率与CEA等肿瘤标志物水平负相关（P＜0.05），子宫内膜癌患者的Ki-67阳性表达率与CEA等肿瘤标志物水平正相关（P＜0.05）；不同年龄的患者间DAPK、Ki-67的表达无差异，高分化、无淋巴结转移、TNM分期为Ⅰ+Ⅱ期的子宫内膜癌患者的DAPK阳性表达率较高（P＜0.05），高分化、无淋巴结转移、TNM分期为Ⅰ+Ⅱ期的子宫内膜癌患者的Ki-67阳性表达率较低（P＜0.05）。结论:DAPK蛋白、Ki-67蛋白在子宫内膜癌患者中存在异常表达，且其表达与患者的病情或者细胞分化、淋巴结转移等具有密切关系。

Expressions of DAPK protein and Ki-67 protein in endometrial carcinoma and their correlations with clinicopathological features

Objective:To investigate the correlation between DAPK protein and Ki-67 protein in endometrial carcinoma and its mechanism.Methods:From June 2012 to October 2016 with endometrial cancer patients in our hospital treatment of 150 study,and 150 cases of endometrial hyperplasia patients (control group) were detected by immunohistochemistry,DAPK protein,Ki-67 protein,expression by automatic biochemical assay of serum CEA,CA19-9 and CA125 etc.The relationship between the expression of DAPK protein,Ki-67 protein and clinical pathological characteristics of endometrial carcinoma was analysed.Results:Endometrial carcinoma tissue in DAPK protein was lower than that of control group (P＜0.05),the expression rate of Ki-67 was higher than that in the benign group (P＜0.05).In endometrial cancer group，CEA,CA19-9 and CA125 were significantly higher than endometrial hyperplasia group (P＜0.05).In endometrial carcinoma patients,the positive expression rates of DAPK were negatively with CEA tumor markers(P＜0.05),and the positive expression rates of Ki-67 were positively related to the level of tumor markers (P＜0.05).No expression difference occured in different age between patients with DAPK,Ki-67.DAPK positive expression of high differentiation,non-lymph node metastasis,TNM stage Ⅰ+Ⅱ of the uterus endometrial cancer was higher (P＜0.05).Ki-67 positive expression of high differentiation,non-lymph node metastasis,TNM stage Ⅰ+Ⅱ endometrial carcinoma rate was low (P＜0.05).Conclusion:DAPK protein and Ki-67 protein show abnormal expression in patients with endometrial cancer,and its expression was correlated with the patient's condition or cell differentiation,lymph node metastasis has a close relationship,have a potential value for the development of endometrial cancer.