外阴鳞状细胞癌肿瘤干细胞的分选和鉴定

目的:分离外阴鳞状细胞癌A-431细胞系中CD44+的细胞亚群，鉴定其具有肿瘤干细胞特性。方法:采用流式细胞术分选出CD44免疫荧光抗体标记的CD44+细胞亚群，检测CD133、CD34蛋白在CD44+和CD44-中表达的差异，检测CD44+及CD44-细胞亚群的细胞周期。结果:A-431细胞系中CD44+细胞亚群约占4%，胚胎干细胞因子CD133、CD34在CD44+和CD44-的VSCC中表达存在显著性差异，主要在细胞膜上表达，CD44+的肿瘤细胞亚群97.69%处于G1期，CD44-的肿瘤细胞亚群61.12%处于S期。结论:在 A-431细胞系中 CD44 可以作为分选肿瘤干细胞的特异性表面标志物，CD133、CD34 在 CD44+的肿瘤细胞高表达，可能成为分选外阴鳞癌肿瘤干细胞的特异性表面标志物，CD44+肿瘤细胞具有典型的干细胞增殖特点。

Separation and characterization of cancer stem like cells in vulvar squamous cell carcinoma

Objective:To isolate the cell subsets of CD44+ from A-431 cell line，to prove that it has the characteristics of cancer stem cells.Methods:To isolate the cell subsets of CD44+ by flow cytometry.To detect the expression of CD133，CD34 protein，comparing protein expression in the CD44+ and CD44- cell population.To detect the cell cycle of the CD44+ cell population and CD44- cell populations.Results:Cell subsets of CD44+ in A-431 cells accounted for about 4%.CD44+ cells differentially expressed the CD133，CD34 at protein levels，mainly expressed on the cell membrane.97.69% CD44+ cancer cells lied in G1 period，61.12% CD44-cancer cells lied in S period.Conclusion:CD44 can become specificity surface marker for isolating cancer stem cells in A-431.The expression of CD133 and CD34 is higher in CD44+ cells，and CD133，CD34 may become specificity surface markers for isolating CSC in VSCC.CD44+ cancer cells have typical characteristics of stem cells proliferation.