| 柴胡皂苷D抑制人结直肠癌细胞SW480增殖的分子机制探讨 |
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| 引用本文： | 党 锋1,杨坤荣1,李 峰2,韩新平1. 柴胡皂苷D抑制人结直肠癌细胞SW480增殖的分子机制探讨[J]. 现代肿瘤医学, 2018, 0(14): 2159-2162. DOI: 10.3969/j.issn.1672-4992.2018.14.005 |
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| 作者姓名： | 党 锋1 杨坤荣1 李 峰2 韩新平1 |
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| 作者单位： | 1.渭南市富平县第二人民医院普通外科，陕西 渭南 711799；2.西安交通大学第二附属医院泌尿外科，陕西 西安 710004 |
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| 摘 要： | 目的:研究柴胡皂苷D（saikosaponin-D，SSD）抑制人结直肠癌细胞SW480增殖的分子机制。方法:Western blot检测SSD对AMPK信号通路相关蛋白（p-AMPKα和p-ACC）表达的影响;Western blot检测siRNA敲低AMPKα后SSD对AMPK信号通路相关蛋白表达的影响;MTT和细胞计数实验研究siRNA敲低AMPKα后SSD对SW480细胞增殖的影响。结果:SSD处理后，p-AMPKα和p-ACC的表达均增高（P＜0.05）;siRNA敲低AMPKα后SSD对p-AMPKα和p-ACC的上调作用被抑制（P＜0.05）;SSD抑制细胞的增殖（P＜0.05），但AMPKα被敲低后SSD对SW480细胞的增殖抑制作用被抑制（P＜0.05）。结论:SSD通过激活AMPK信号通路抑制人结直肠癌细胞SW480的增殖。
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| 关 键 词： | 柴胡皂苷D 人结直肠癌 细胞增殖 AMPK |
The molecular mechanism disscussion on saikosaponin-D inhibits proliferation of colorectal cancer cell line SW480 |
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| Dang Feng1,Yang Kunrong1,Li Feng2,Han Xinping1. The molecular mechanism disscussion on saikosaponin-D inhibits proliferation of colorectal cancer cell line SW480[J]. Journal of Modern Oncology, 2018, 0(14): 2159-2162. DOI: 10.3969/j.issn.1672-4992.2018.14.005 |
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| Authors: | Dang Feng1 Yang Kunrong1 Li Feng2 Han Xinping1 |
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| Affiliation: | 1.Department of General Surgery,The Second People's Hospital of Fuping County,Shaanxi Weinan 711799,China;2.Department of Urology,The Second Affiliated Hospital of Xi'an Jiaotong University,Shaanxi Xi'an 710004,China. |
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| Abstract: | Objective:To study the molecular mechanism of saikosaponin-D（SSD)-inhibited proliferation inhuman colorectal cancer cell line SW480.Methods:Using Western blot to check the impacts of SSD treatment on AMPK signaling pathway with/without AMPKα knockdown by its specific siRNA.Utilizing MTT assay and cell counting assay to check the influence of SSD on cell proliferation with/without AMPKα knockdown.Results:SSD treatment upregulated the expression of p-AMPKα and p-ACC（P＜0.05).AMPKα knockdown suppressed the upregulation expression ability of SSD on p-AMPKα and p-ACC（P＜0.05).SSD inhibited the proliferation of SW480（P＜0.05),AMPKα knockdown attenuated the proliferation inhibition capacity of SSD on SW480（P＜0.05).Conclusion:SSD inhibited proliferation of colorectal cancer cell line SW480 via AMPK activation. |
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| Keywords: | saikosaponin-D colorectal cancer proliferation AMPK |
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