盐霉素增加鼻咽癌CNE-2细胞放疗敏感性的实验研究

目的:探讨盐霉素（Sal）对鼻咽癌CNE-2细胞的放疗增敏作用及其可能的机制。方法:CCK8测定不同浓度盐霉素、不同剂量放疗及联合应用对CNE-2细胞增殖的抑制作用；平板克隆实验观察盐霉素联合放疗后细胞的克隆形成率；Hoechst33258染色观察细胞凋亡；流式细胞术检测细胞凋亡率及细胞周期的变化情况。结果:CCK8结果显示，盐霉素和放疗对鼻咽癌CNE-2细胞的生长有显著的抑制作用，呈浓度和剂量依赖性，且联合组抑制作用强于单纯药物组或放疗组。平板克隆实验显示盐霉素联合放疗后能显著降低细胞的克隆形成率；Hoechst33258染色显示盐霉素联合放疗治疗后细胞凋亡现象更明显；细胞流式术检测显示联合组较单纯药物组或放疗组凋亡率增加，盐霉素对放疗具有增敏作用。药物组和放疗组均能使G2/M期细胞比例增加，两者联合效果更明显。结论:盐霉素能增加人鼻咽癌CNE-2细胞的放疗敏感性，其作用机制可能与周期阻滞及其凋亡诱导相关。

Synergistic radiosensitization of salinomycin on nasopharyngeal carcinoma CNE-2 cell

Objective:To investigate the effect of salinomycin on radiosensitization of nasopharyngeal carcinoma CNE-2 cell line．Methods:CCK8 method was used to detect the effect of combination treatment with salinomycin and irradiation on proliferation.Plate clone assay was used to measure the clone formation rate.Hoechst33258 staining was used to perform morphological assessment of apoptotic cells.Cell cycle distribution and apoptosis were analyzed by flow cytometry．Results:CCK8 assay showed exposure to combination of salinomycin and irradiation resulted in decline of CNE-2 cell viability rate in concentration dose or dose-dependent manner and combination treatment had an additive inhibitory effect.Plate clone assay showed the combination treatment resulted in reduction of clone formation.In Hoechst33258 staining assay,apoptotic bodies of NPC cells added in response to the combination treatment.FCM assay showed the combination treatment induced strongly augmented number of apoptotic cells induced apoptosis and blocked cell cycle G1／S phase.Conclusion:Our study shows that salinomycin may enhance radiosensitization on CNE-2 cells．The radiosensitivity enhancement of may be related to apoptosis and cell cycle arrest．