岩黄连水提物对肝癌HepG2细胞增殖和迁移能力的影响及其可能机制

目的 观察岩黄连水提物对原发性肝癌HepG2细胞增殖和迁移能力的影响，并探讨其可能的作用机制。方法 将含不同浓度（0.4 mg/mL、0.8 mg/mL、1.6 mg/mL）的岩黄连水提物100 mL作用于肝癌 HepG2 细胞，以等量未处理培养基培养的肝癌HepG2细胞为对照组。采用CCK-8法检测肝癌HepG2细胞的增殖能力，Transwell法检测细胞迁移能力，Western blot和荧光定量PCR分别检测NF-κBp65蛋白和mRNA的表达水平。结果CCK-8实验结果显示，24 h、48 h和72 h不同浓度岩黄连水提物作用肝癌HepG2细胞的增殖抑制率组内差异均有统计学意义（P<0.01），且0.8 mg/mL、1.6 mg/mL干预的细胞增殖抑制率均高于0.4 mg/mL（P<0.01）。Transwell实验结果显示，不同浓度岩黄连水提物作用肝癌HepG2细胞48 h后的细胞迁移数均低于对照组（P<0.001），且可上调NF-κBp65蛋白和mRNA的表达（P<0.001）。结论 岩黄连水提物能抑制肝癌HepG2细胞增殖、迁移能力，其作用机制可能与上调NF-κBp65表达有关。

Effect of corydalis saxicola bunting aqueous extract on proliferation and migration of hepatocellular carcinoma HepG2 cells and its possible mechanism

Objective To investigate the effect of corydalis saxicola bunting aqueous extract on hepatocellular carcinoma HepG2 proliferation and migration and its possible mechanism. Methods The culture medium containing different concentrations（0.4 mg/mL 0.8 mg/mL 1.6 mg/mL） of corydalis saxicola bunting aqueous extract was applied to hepatocellular carcinoma HepG2 cells. The proliferation of HepG2 cells was assayed using the cell counting kit-8（CCK-8），and invasion was detected using the Transwell test. The expression of NF-κBp65 was detected by real-time PCR and Western blot analysis. Results The results of CCK-8 showed that the inhibition rate of HepG2 cells in different concentra-tions of corydalis saxicola bunting aqueous extract at 24 h，48 h，and 72 h was statistically significant （P<0.05），and the cell prolifer-ation in-hibition rate of 0.8 mg/mL and 1.6 mg/mL was higher than 0.4 mg/mL（P<0.05）. The results of Transwell experiments showed that the cell migration of HepG2 cells treated with different concentrations of Rhizoma Coptidis aqueous extracts for 48 h was lower than that of the control group （P<0.05），and the expression of NF-Bp65 protein and mRNA was up-regulated （P<0.05）. Conclusions Corydalis saxicola bunting aqueous extract inhibits proliferation，migration of HepG2，and the mechanism of it may do so by up-regulating NF-κBp65 expression.