食管鳞癌组织中TSLC1基因启动子甲基化状态检测的临床意义

目的:探讨肺癌抑癌基因1（TSLC1）在食管鳞癌组织中基因启动子甲基化状态及其与临床病理参数的关系。方法:应用甲基化特异性PCR检测TSLC1基因启动子在98例食管鳞癌组织及相应癌旁组织中的甲基化状态并结合临床病理资料进行分析。结果:在食管鳞癌组织中TSLC1基因甲基化阳性率（55.1%，54/98）显著高于癌旁组织（4.1%，4/98）（P<0.01）。TSLC1甲基化与食管鳞癌临床分期和浸润深度显著相关（P=0.035和0.001）。结论:TSLC1基因甲基化可能是参与食管鳞癌发展的重要分子事件。

Clinical significance of TSLC1 gene promoter hypermethylation in human esophageal squamous cell carcinoma

Objective:To investigate the methylation status of the tumor suppressor in lung cancer 1 (TSLC1) gene promoter in esophageal squamous cell carcinoma.Methods:Methylation-specific PCR was performed to observe TSLC1 gene promoter methyation in 98 cases of esophageal squamous cell carcinoma tissues companied with their adjacent non-cancerous tissues.The relationship between TSLC1 methylation and clinical pathological parameters was also analyzed.Results:Methylated TSLC1 gene promoter was more frequently observed in primary tumors than in adjacent non-cancerous tissues (P<0.01).TSLC1 methylation was significantly correlated with TNM staging (P=0.035) and tumor depth (P=0.001).Conclusion:TSLC1 methylation may possess a significant function in the development of human esophageal squamous cell carcinoma.