miR-124对宫颈癌Hela细胞放射敏感性的影响及其机制探讨

Effect of miR-124 on the radiosensitivity of cervical cancer Hela cells and its mechanism

Objective:To investigate the effect of miR-124 on radiosensitivity of cervical cancer Hela cells and its mechanism.Methods:The miR-124 mimics and miR-124 inhibitor were transfected into Hela cells.Cell cycle experiment showed that miR-124 could influence cell cycle.The important role of miR-124 in radiation sensitivity of Hela cells was confirmed by colony-formation assay and cell apoptosis assay.The target gene of miR-124 was confirmed by luciferase reporter assay,RT-PCR and Western blot.Results:The percentage of G0/G1 phase cells in miR-124 mimics group was higher than that in the control group，and the percentage of cells in S phase was lower than that of the control group(all P＜0.05).Overexpression of miR-124 could inhibit Hela cells ability of colony formation，and SER was 1.49.Flow cytometric analysis showed that the overexpression of miR-124 had an effect on the proliferation and apoptosis of cervical cancer cells after irradiation（P＜0.05).Inhibition of miR-124 would lead to the contrary results（P＜0.05).Luciferase reporter gene test,RT-PCR and Western blot experiments showed that STAT3 was a direct target gene of miR-124（all P＜0.05）.Conclusion:miR-124 may contribute to enhancing radiosensitivity of cervical cancer Hela cells by targeting STAT3.