唾液腺黏液表皮样癌干细胞的分离、鉴定及生物学特征分析

目的: 筛选出唾液腺黏液表皮样癌细胞的特异基因,进一步了解肿瘤干细胞的成瘤机制。方法: 通过有限稀释法对肿瘤细胞行单细胞培养,观察肿瘤干细胞单克隆生长的情况。应用免疫磁珠分离技术,确定唾液腺黏液表皮样癌干细胞的相对特异性表面标志。采用SPSS 11.5软件包对数据进行统计学分析。结果: CD133在唾液腺黏液表皮样癌细胞株M3SP2单克隆细胞株中表达较高,具有显著差异（P<0.05）。结论: CD133可能是唾液腺黏液表皮样癌细胞株M3SP2单克隆细胞株的表面特异性抗原标记物或组合之一。

Isolation,identification and biological characteristics of the stem cells from mucoepidermoid carcinoma of the salivary gland

PURPOSE: To study the specific genes of mucoepidermoid carcinoma of mucus - like cancer cells of the salivary gland, and to further understand the mechanism of tumor stem cells. METHODS: Single cell culture of tumor cells was performed by limited dilution method. The growth of tumor stem cells was observed. The relative specific surface markers of salivary gland mucoepidermoid carcinoma stem cells were determined by immunomagnetic separation technology. The data were analyzed with SPSS 11.5 software package. RESULTS: The expression of CD133 was higher in the M3SP2 cell line, and the high expression of CD133 was significantly different（P<0.05）. CONCLUSIONS: CD133 may be one of the surface specific antigen markers of M3SP2 monoclonal cell lines in salivary gland mucoepidermoid carcinoma cell line.