利用网络分析预测As2O3治疗结直肠癌的靶基因

目的:探究三氧化二砷（arsenic trioxide，As2O3）作用于结直肠癌的靶基因。方法:利用pharmGKB数据库和drugbank5.0数据库分别提取三氧化二砷的直接靶基因，利用STRING10.5数据库筛选与三氧化二砷已知靶基因互相作用的蛋白，利用webGestalt数据库对互作蛋白进行通路分析和GO注释，运用cBioProtal数据库和The Human Protein Atlas 数据库对重点通路的核心基因进行变异和表达分析。结果:pharmGKB数据库和drugbank5.0数据库共提取出11个直接靶基因，STRING10.5数据库筛选出384个互作蛋白，webGestalt数据库的GO注释这384个靶基因主要集中于19类分子功能，在前10条通路中筛选出3条与结直肠癌有关的通路，3个核心基因在结直肠癌中存在点突变和基因缺失，其中NF-κB1和RELA在结直肠癌组织中存在高表达。结论:三氧化二砷可能通过 NF-κB信号通路治疗结直肠癌。

Using network analysis to predict the target genes of As2O3 in the treatment of colorectal cancer

Objective:To explore the target genes of As2O3 in therapy of colorectal cancer (CRC).Methods:The direct target genes were extracted by pharmGKB database and drugbank5.0 database.Direct protein targets associated genes were filtrated by STRING10.5 database.KEGG pathway and GO analysis of interactive protein were operated by webGestalt database.Hub genes from important pathways were analyzed by cBioProtal database and the Human Protein Atlas database.Results:11 direct target genes were found from pharmGKB and drugbank5.0.384 direct protein targets associated genes were identified for As2O3 by STRING10.5.And 19 molecular functions and top 10 pathways were explored by webGestalt.Finally，3 hub genes were found out.Moreover，3 hub genes have mutation and gene deep deletion in CRC，of which NF-κB1 and RELA are highly expressed in CRC tissues.Conclusion:As2O3 may be used to treat CRC through the NF-κB signaling pathway.