高效液相色谱法同时测定蒙药阿那日-4散中3种成分的含量

目的：建立蒙药阿那日-4散（石榴、肉桂皮、白豆蔻、荜茇）中有效成分没食子酸、桂皮醛和胡椒碱的含量测定方法。方法：采用反相高效液相色谱法以Promosil C₁₈（4.6 mm×250 nm，5μm）为色谱柱；以乙腈-1%冰乙酸水溶液为流动相，梯度洗脱；流速为1.0 mL·min^-1；柱温为30℃；检测波长为290 nm。结果：没食子酸在0.08~0.4μg·mL^-1（r=0.9998）、桂皮醛在0.56~2.81μg·mL^-1（r=0.9996）、胡椒碱在0.52~2.59μg·mL^-1（r=0.9997）范围内与峰面积呈良好的线性关系；其平均回收率（n=6）分别为99.5%（RSD=2.3%）、99.3%（RSD=2.8%）和98.5%（RSD=2.2%）；6批样品中上述3种成分平均含量范围分别为0.198~0.241，2.44~2.61，1.41~1.54μg·g^-1。结论：所建立方法操作简便、稳定可靠、专属性强、准确度及重复性较好，为蒙药阿那日-4散质量检测提供依据。

Simultaneous determination of 3 components in Anari-4 by high performance liquid chromatography

OBJECTIVE To develop an HPLC method for simultaneous determination of gallic acid, cinnamaldehyde and piperine in Anari-4.METHODS Promosil C₁₈ (4.6 mm×250 nm, 5 μm) was used with acetonitrile and acetic acid water solution as mobile phase in a gradient mode at the flow rate of 1.0 mL·min^-1. The detection wavelength was 290 nm and the temperature of column was set at 30℃.RESULTS A good linear range of gallic acid was 0.08-0.45 μg·mL^-1 by HPLC (r=0.999 8), the average recovery was 99.5% (RSD was 2.3%, n=6). A good linear range of cinnamaldehyde was 0.56-2.81 μg·mL^-1 by HPLC (r=0.999 6), the average recovery was 99.3% (RSD was 2.8%, n=6). A good linear range of piperine was 0.52-2.59 μg·mL^-1 by HPLC (r=0.999 7), the average recovery was 98.5% (RSD was 2.2%, n=6). The average contents of gallic acid, cinnamaldehyde and piperine in six batches of samples were 0.198-0.241, 2.44-2.61 and 1.41-1.54 μg·g^-1, respectively.CONCLUSION The method is simple, stable, accurate, reliable, and can provide the basis for quality control of Anari-4.