雷帕霉素对高糖诱导人晶状体上皮细胞氧化应激的保护作用

目的　探讨雷帕霉素对高糖诱导的人晶状体上皮细胞（human lens epithelial cells，HLECs）氧化应激的影响。方法　建立高糖诱导的HLECs氧化损伤模型，用不同浓度(10 nmol·L^-1、100 nmol·L^-1)雷帕霉素干预，CCK-8比色法检测细胞存活率，倒置显微镜观察细胞形态并拍照，H_2DCFDA检测细胞内活性氧水平，Western Blotting检测各组凋亡相关蛋白Bcl-2及Bax 的表达情况。结果　CCK-8检测结果显示，用10 nmol·L^-1、100 nmol·L^-1雷帕霉素处理后，HLECs存活率分别提高到62.86%±3.45%和77.47%±4.21%，与高糖组（42.32%±3.10%）比较差异均有统计学意义（均为P<0.05）；H_2DCFDA荧光探针染色结果显示，雷帕霉素处理后HLECs内活性氧生成量下降，氧化损伤细胞减少；此外，雷帕霉素可以抑制高糖诱导的HLECs内凋亡蛋白Bax、Bcl-2的表达，抑制效应与剂量正相关。结论　雷帕霉素可以抑制高糖诱导的HLECs氧化损伤及凋亡的发生，对糖尿病性白内障具有预防作用。

Rapamycin protects human lens epithelial cells against oxidative stress induced by high glucose

Objective　To investigate the effects of rapamycin on human lens epithelial cells （HLECs） against high glucose-induced oxidative stress.Methods　A oxidative damage model of HLECs induced by high glucose was established，and intervention with different concentrations of rapamycin for the cells was performed for detecting the survival rate by CCK-8 assay，observing cell morphology by inverted microscope，and measuring the level of intracellular reactive oxygen species （ROS） by H2DCFDA staining，as well as determining the expression of apoptosis related Bcl-2 and Bax proteins by Western blot in all groups.Results　CCK-8 results showed that the survival rate of HLECs was increased to 62.00%±3.74% and 79.57%±5.26% after treated with 10 nmol·L^-1 and 100 nmol·L^-1 rapamycin，and the difference was statistically significant when compared with the high glucose group （42.32%±3.10%） （all P<0.05）；H2DCFDA fluorescent probe staining demonstrated that rapamycin could suppress ROS generation in HLECs and alleviate oxidative damage to cells；besides，rapamycin could downregulate the expression of Bcl-2 and Bax protein in HLECs and the inhibitory effects were positively correlated with the concentration of rapamycin.Conclusion　Rapamycin can inhibit high glucose-induced oxidative damage and apoptosis in human HLECs，which provides a prevention effect for diabetic cataract.