高效液相色谱法同时测定夜香牛颗粒中绿原酸和木犀草苷的含量

目的：建立夜香牛颗粒中绿原酸和木犀草苷含量分析的方法。方法：采用FORTIS-C₁₈柱（4.6 mm×250 mm，5 μm），以乙腈-0.05%KH₂PO₄溶液为流动相，采用梯度洗脱，流速1.0 mL·min^-1，柱温30 ℃，检测波长330 nm。 结果：绿原酸在4.82~96.30 μg·mL^-1、木犀草苷在1.62~32.40 μg·mL^-1范围内有良好线性关系（r=0.999 9），平均加样回收率分别为98.8%和98.4%，RSD分别为1.07%和1.64%。结论：本方法灵敏度高、准确、重复性好、专属性强，可用于夜香牛颗粒的质量控制。

Determination of chlorogenic acid and luteoloside in Vernonia cinerea granules by HPLC

OBJECTIVE To establish a method for determination of chlorogenic acid and luteoloside in Vernonia cinerea granules. METHODS The separation was performed on the Fortis-C₁₈ column (4.6 mm×250 mm,5 μm) with acetonitrile and 0.05% KH₂PO₄ as the mobile phase at the flow rate of 1.0 mL·min^-1. The column temperature was 30℃, and the UV detector was set at 330 nm. RESULTS There was good linearity between the peak area and concentration in the ranges of 4.82-96.30 μg·mL^-1 and 1.62-32.40 μg·mL^-1 (r=0.999 9) for chlorogenic acid and luteoloside, respectively. The average recovery rates were 98.8% and 98.4%. RSD were 1.07% and 1.64%. CONCLUSION The method is highly sensitive, accurate, repeatable and specific, so it is an accurate method for Vernonia cinerea granule quality control.