胰腺癌患者血循环miR-429的表达及其临床意义

目的:检测胰腺癌患者血浆miR-429表达水平，分析其表达与胰腺癌临床病理学特征的关系。方法:定量PCR检测BXPC-3、PANC-1细胞株以及3例胰腺癌组织中miR-429的表达水平。收集未经治疗的37例胰腺癌患者的血浆标本，采用实时定量PCR法检测miR-429表达水平，收集胰腺癌患者的临床病理学参数(年龄、临床分期、分化程度、组织类型及淋巴结转移情况)，比较不同miR-429水平的临床病理参数分布差异。结果:相较于正常胰腺细胞、胰腺组织，miR-429在胰腺癌细胞株和癌组织中均处于低表达水平(P<0.05)。miR-429表达与肿瘤大小、TNM临床分期、神经浸润以及生存期密切相关，均有统计学差异(P<0.05)。结论:循环血miR-429的表达可能成为胰腺癌早期诊断、预后判断的指标。

The expression of miR-429 in patients with pancreatic cancer and clinical significance

Objective:To investigate the expression of miR-429 in pancreatic cancer and explore the relationship between the expression of miR-429 and its clinical pathology features.Methods:The expression of miR-429 in BXPC-3,PANC-1 cells and pancreatic cancer tissues were inspected by Real-time PCR.Collect serum from 37 patients with pancreatic cancer.Real-time PCR was conducted to measure miR-429 expression in the serum of pancreatic cancer patients.The relationship between miR-429 expression and pathological features were analyzed.Results:miR-429 was observed down-regulated in pancreatic cancer cell lines,tissue and in patient's serum(P<0.05).The statistical analysis suggested that miR-429 was associated with tumor size,TNM stage,nerves infiltration and survival(P<0.05).Conclusion:The expression of miR-429 in serum could be a new biomarker for pancreatic cancer early diagnosis and predictor of prognosis.