下调Ets2表达对肾癌786-0细胞迁移及侵袭的影响

目的:探讨E26转录因子2（E-twenty six 2,Ets2）对肾癌细胞786-0迁移、侵袭的影响及其机制。方法:设计并合成特异性siRNA下调肾癌细胞系786-0中Ets2的表达；并利用划痕实验及Transwell实验检测其迁移及侵袭能力的变化；通过Western blot检测下调Ets2表达后基质金属蛋白酶2（MMP-2）及MMP-9表达水平变化。结果:siRNA能有效下调肾癌786-0细胞系中Ets2的表达。细胞划痕实验结果显示下调Ets2表达后，细胞迁移能力减弱（P＜0.01）；Transwell实验中NC组穿膜细胞数为（43.80±3.99）个，而siRNA1组为（23.30±4.24）个(P＜0.01)， siRNA2组为（24.20±3.29）个（P＜0.01），细胞侵袭能力减弱。Ets2-siRNA介导的Ets2基因沉默下调了786-0细胞中MMP-2及MMP-9的表达的同时上调了基质金属蛋白酶组织抑制剂1（TIMP1）和TIMP2的表达。结论:Ets2可通过调节MMPs及TIMPs的表达影响肾癌细胞的迁移及侵袭能力。

Influence of siRNA targeting Ets2 gene on migration and invasion of renal cell carcinoma cell 786-0

Objective:To investigate the mechanism of E-twenty six 2（Etx2) promote 786-0 cells' migration and invasion.Methods:Design and synthesize the specific siRNA that can down-regulate the expression of Ets2 in 786-0 cells.siRNAs were transfected into 786-0 cells and the effect was detected by Western blot.Migration and invasion ability were tested by the wound scratch assay and the Transwell chamber assay.The expression levels of matrix metalloproteinases-2（MMP-2),MMP-9,tissue inhibitor of matrix metalloproteinases 1(TIMP1)， TIMP2 were detected by Western blot.Results:siRNA can effectively down-regulate the expression of Ets2 in 786-0 cells.Down-regulation of Ets2 expression can suppress the cell migration and invasion capacity.The cell number in negative control group,siRNA1 group,siRNA2 group was 43.80±3.99,23.30±4.24 and 24.20±3.29 respectively（P＜0.01).In addition,the expressions of MMP-2 and MMP-9 were decreased,while the expression of TIMP1 and TIMP2 were increased in siRNA groups.Conclusion:Ets2 may regulate MMP-2 and MMP-9 to promote migration and invasion in 786-0 cells.Ets2 may be associated with the development and metastatic in renal cell carcinoma.