黄芪多糖抑制IL-1β表达及改善脓毒症小鼠心功能研究

目的 探讨黄芪多糖对脓毒症小鼠心肌成纤维细胞IL-1β表达水平及心功能的影响。方法 取2~3月龄C57BL/6小鼠,设立对照组。以腹腔注射脂多糖(lipopolysaccharidi,LPS)建立脓毒症模型,设立脓毒症组和黄芪多糖(astragalus polysaccharides,APS)处理组,6 h后酶联免疫吸附法(ELISA)检测小鼠外周血IL-1β蛋白表达,24 h后超声心动图检测小鼠心脏功能。取对照组C57BL/6小鼠,体外分离培养小鼠心肌成纤维细胞,设立对照组、LPS处理组和黄芪多糖低、中、高浓度(1、10、100 mg/L)+LPS处理组。采用ELISA检测细胞上清液中IL-1β,采用免疫印迹法检测Pro-IL-1β及IL-1β蛋白。结果 脓毒症小鼠心脏功能较对照组明显减退(P<0.05),黄芪多糖处理组较未处理的脓毒症小鼠心脏功能明显改善(P<0.05)。脓毒症组外周血IL-1β蛋白较对照组明显升高(P<0.05),黄芪多糖处理组的脓毒症小鼠IL-1β明显降低(P<0.05); LPS或联合三磷酸腺苷(ATP)诱导的心肌成纤维细胞与对照组相比,LPS组(LPS 1 ng/mL)诱导心肌成纤维细胞Pro-IL-1β及IL-1β蛋白明显增加(P<0.05),而黄芪多糖处理组较LPS组相比,Pro-IL-1β及IL-1β蛋白表达明显减少(P<0.05)。结论 黄芪多糖对脓毒症小鼠心肌成纤维细胞IL-1β蛋白表达有抑制作用,改善脓毒症引起的心脏功能障碍。

Astragalus polysaccharides inhibits the expression IL-1β protein and improves the cardiac function of mice with sepsis

Objective To investigate the effects of astragalus polysaccharides (APS) on the expression of IL-Iβ protein in myocardial fibroblasts and cardiac function of mice with sepsis. Methods In vivo, the sepsis model was established through the peritoneal injection of lipopolysaccharidi (LPS). Mice (C57BL/6) of 2-3 months were divided into three groups: control, sepsis, and sepsis treated with APS. Mouse circulating IL-1β was determined by enzyme linked immunosorbent assays (ELISA) after 6 hours, mouse myocardial function was detected by echocardiography after 24 hours; In vitro, cardiac fibroblasts (CF) were isolated and cultured from mice. The CF were divided into control, LPS (1 ng/mL),and LPS with APS (1,10,and 100 mg/L) groups. The supernatant IL-1β of CF was determined by ELISA. Interleukin (IL)-1β and pro-IL-1β protein was determined by Western blotting. Results As compared with the control group, the myocardial function in mice with sepsis was significantly reduced (P<0.05), which was improved by APS treatment (P<0.05). Circulating IL-1β was increased in mice with sepsis as compared with the control mice (P<0.05). That was attenuated by the treatment of the septic mice with APS (P<0.05); as compared with the control (P<0.05) CF, the treatment of LPS (1 ng/mL) or LPS with ATP increased the pro-IL-1β and IL-1β protein expression in CF, while the treatment of APS deceased the pro-IL-1β and IL-1β protein expression in CF as compared with the treatment of LPS (P<0.05). Conclusions APS attenuates myocardial dysfunction in mice with sepsis by inhibiting the expression of IL-1β in the CF .