结肠癌细胞中Ese-3对树突状细胞分化成熟的影响

目的:利用体外共培养实验，观察结肠癌细胞SW480中上皮特异性转录因子Ese-3表达水平的改变对树突状细胞（dendritic cell，DC）分化成熟的影响。方法:利用慢病毒系统获得过表达Ese-3的结肠癌细胞SW480（SW480 Ese-3）及其对照细胞株（SW480 NC）。利用磁珠分选方式，从人外周血单个核细胞（PBMC）中获得CD14+细胞，经rhGM-CSF、rhIL-4刺激获得不成熟的DC（iDC）。通过Transwell小室将SW480 Ese-3和SW480 NC细胞分别与iDC进行间接共培养。流式细胞术检测共培养后的DC细胞中HLA-DR、CD14、CD83、CD86 的表达。ELISA检测细胞培养上清中IL-12p70的水平。结果:SW480 Ese-3/DC共培养组DC细胞表面标志物HLA-DR、CD83、CD86水平，较SW480 NC/DC共培养组升高，CD14水平降低；SW480 Ese-3/DC共培养组上清中IL-12p70水平较对照组升高。结论:在体外共培养实验中，SW480细胞过表达Ese-3可促进DC细胞分化成熟。

Ese-3 regulates the activation of DC in colorectal cancer cell

Objective:To explore the effect of epithelium-specific Ets-3（Ese-3) in colorectal cancer cells on the differentiation and maturation of dendritic cells（DCs) in vitro co-culture experiment.Methods:lentivirus-mediated biological transfection technique was used to build a stable expression of Ese-3 gene in SW480 cell line（SW480 Ese-3) and its relative controls（SW480 NC).CD14+ cells were obtained from human peripheral blood mononuclear cells（PBMC) by magnetic bead sorting,and induced to produce iDC by IL-4 and rhGM-CSF.The iDC were stimulated by TNF-α,and co-cultured with SW480 Ese-3 and its control cell line SW480 NC respectively by Transwell chamber.The expression of HLA-DR,CD14,CD83 and CD86 in co-cultured DC cells was detected by flow cytometry.The level of IL-12p70 in supernatant of cell culture was detected by ELISA.Results:The levels of HLA-DR,CD83 and CD86 on DCs in the SW480 Ese-3/DC co-culture group were higher than those in SW480 NC/DC co-culture group and the level of CD14 was decreased.The level of IL-12p70 in SW480 Ese-3/DC culture supernatant were higher than those in the control group.Conclusion:In vitro co-culture experiments,SW480 cells overexpressing Ese-3 can promote the differentiation and maturation of DC cells.