Association and histological characteristics of endplate injury and intervertebral disc degeneration in a rat model |
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| Institution: | 1. Department of Orthopedics, Shanghai Tenth People''s Hospital, Tongji University School of Medicine. No.301 Yanchang Middle Road, Shanghai 200072, China;2. Department of Orthopedics, Shanghai East Hospital, Tongji University School of Medicine, China. No. 150 Jimo Road, Shanghai 200120, China;3. Department of Bone and Soft Tissue Tumors, Tianjin Medical University Cancer Institute and Hospital. No.1 North Huanhu West Road, Tianjin 300060, China;1. Department of Veterinary Surgery, Paulista State University Júlio de Mesquita Filho, Access Road Highway Professor Paulo Donato Castelane Castelane S / N, Vila Industrial, [Zip Code]: 14884-900, Jaboticabal-SP, Brazil;2. Institute of Biomedical Sciences, Federal University of Uberlândia, Avenue. João Naves de Ávila, 2121 - Santa Monica, [Zip Code]: 1720, 38400-902, Uberlândia-MG, Brazil;3. Institute of Biology, Campinas State University, Street Monteiro Lobato, 255. [Zip Code]: 1308-862, Campinas-SP, Brazil;4. Veterinarian, Federal University of Uberlândia, Avenue Mato Grosso, 3289 – Código postal [Zip Code]: 38405-314, Bloco 2S - Umuarama, Uberlândia - MG, Brazil;5. Department of Veterinary Surgery, Federal University of Uberlândia, Av. Mato Grosso, 3289 - [Zip Code]: 38405-314, Bloco 2S - Umuarama, Uberlândia - MG, Brazil;1. Department of Orthopedics and Rehabilitation, University of Wisconsin-Madison, 1685 Highland Avenue, 6th Floor, Madison, WI, 53705, USA;2. Department of Biostatistics and Medical Informatics, University of Wisconsin-Madison, 610 Walnut Street, Madison, WI, 53726, USA;3. Department of Orthopedic Surgery, University of Nebraska/Children''s Hospital & Medical Center, 8200 Dodge Street – Pavilion, Omaha, NE, 68114, USA;1. Department of Surgery, University of North Carolina, Chapel Hill, North Carolina 27599, USA;2. Department of Surgery, Kamuzu Central Hospital, Lilongwe Malawi |
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| Abstract: | IntroductionThe purpose of this study was to construct a rat caudal vertebral body fracture model and to analyze the association and histological characteristics of vertebral body fracture with endplate injury and adjacent intervertebral disc degeneration.Materials and methodsThis study included 144 clean-grade male Sprague-Dawley rats, which were randomly divided into a control, middle vertebral body injury (MI), and endplate injury (EI) groups. A vertebral body fracture with or without endplate injury was developed by either drilling a hole in the middle of a rat caudal vertebral body to create a fracture with an intact endplate or drilling a hole in the vertebral body near the intervertebral disc to create a vertebral body fracture with endplate injury. The histological differences in the adjacent intervertebral discs of vertebral body fractures with or without endplate injury were detected using imaging, non-specific histological staining, immunohistochemistry and TUNEL assay.ResultsImaging results revealed that the EI group showed a significant decrease in intervertebral space height and intervertebral disc T2 signal over time. Non-specific histological staining revealed that in the EI group, the intervertebral disc was degenerative. Immunohistochemistry indicated that Aggrecan and Collagen-II were decreased and inflammatory factors were increased in the EI group. The TUNEL detection found that apoptosis was significantly increased in the EI group as compared with the MI and control groups.ConclusionIn rat caudal vertebral body fractures, a fracture with endplate injury is more likely to induce or accelerate degeneration of adjacent intervertebral discs. |
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