| Methylation of Gene CHFR Promoter in Acute Leukemia Cells |
| |
| 引用本文： | 龚辉 刘文励 周剑峰 徐慧珍. Methylation of Gene CHFR Promoter in Acute Leukemia Cells[J]. 华中科技大学学报(医学英德文版), 2005, 25(3): 240-242. DOI: 10.1007/BF02828130 |
| |
| 作者姓名： | 龚辉 刘文励 周剑峰 徐慧珍 |
| |
| 作者单位： | DepartmentofHematology,TongjiHospital,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan430030,China |
| |
| 基金项目： | This project was supported by grants from National Natu-ral Sciences Foundation of China (No .30271472) and Na-tional Key Basic Research Development Programme“973”(No .2002CB513100) . |
| |
| 摘 要： | In order to explore whether gene CHFR was inactivated by methylation in leukemia cells, the expression of CHFR was examined before and after treatment with demethylation agent in Molt-4, Jurkat and U937 leukemia cell lines by means of RT-PCR， The methylation of promoter in Moh-4, Jurkat and U937 cells as well as 41 acute leukemia patients was analyzed by MS-PCR. The results showed that methylation of CHFR promoter was inactivated and could be reversed by treatment with a demethylating agent in Molt 4, Jurkat and U937. CHFR promoter methylation was detected in 39 % of acute leukemia patients. There was no difference in incidence of CHFR promoter methylation between acute myeloeytie leukemia and acute lymphocytic leukemia. In conclusion, CHFR is frequently inactivated in acute leukemia and is a good candidate for the leukemia supper gene. By affecting mitotic checkpoint function, CHFR inactivation likely plays a key role in tumorigenesis in acute leukemia. Moreover, the methylation of gene CHFR appears to be a good index with which to predict the sensitivity of acute leukemia to microtubule inhibitors.
|
| 关 键 词： | CHFR 基因甲基化 急性白血病 肿瘤细胞 微管抑制 |
| 收稿时间： | 2005-04-29 |
Methylation of gene CHFR promoter in acute leukemia cells |
| |
| Gong Hui,Liu Wengli,Zhou Jianfeng,Xu Huizhen. Methylation of gene CHFR promoter in acute leukemia cells[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2005, 25(3): 240-242. DOI: 10.1007/BF02828130 |
| |
| Authors: | Gong Hui Liu Wengli Zhou Jianfeng Xu Huizhen |
| |
| Affiliation: | Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
| |
| Abstract: | In order to explore whether gene CHFR was inactivated by methylation in leukemia cells, the expression of CHFR was examined before and after treatment with demethylation agent in Molt-4, Jurkat and U937 leukemia cell lines by means of RT-PCR. The methylation of promoter in Molt-4, Jurkat and U937 cells as well as 41 acute leukemia patients was analyzed by MS-PCR. The results showed that methylation of CHFR promoter was inactivated and could be reversed by treatment with a demethylating agent in Molt-4, Jurkat and U937. CHFR promoter methylation was detected in 39 % of acute leukemia patients. There was no difference in incidence of CHFR promoter methylation between acute myelocytic leukemia and acute lymphocytic leukemia. In conclusion, CHFR is frequently inactivated in acute leukemia and is a good candidate for the leukemia supper gene. By affecting mitotic checkpoint function, CHFR inactivation likely plays a key role in tumorigenesis in acute leukemia. Moreover, the methylation of gene CHFR appears to be a good index with which to predict the sensitivity of acute leukemia to microtubule inhibitors. |
| |
| Keywords: | leukemia CHFR promoter methylation |
| 本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录！ |
