| 应用等位基因特异性PCR和多重差别PCR检测肺癌患者CYP1A1和GSTM1的遗传多态性 |
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| 引用本文： | 陈森清,许林,马国建,吴建中,薛开先. 应用等位基因特异性PCR和多重差别PCR检测肺癌患者CYP1A1和GSTM1的遗传多态性[J]. 癌变．畸变．突变, 1999, 0(3) |
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| 作者姓名： | 陈森清 许林 马国建 吴建中 薛开先 |
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| 作者单位： | 江苏省肿瘤防治研究所 |
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| 摘 要： | 本文对等位基因特异性ＰＣＲ（Ａｌｅｌｅｓｐｅｃｉｆｉｃ，ＡＳ）和多重差别（Ｍｕｌｔｉｐｌｅｘｄｉｆｅｒｅｎｔｉａｌ，ＭＤ）ＰＣＲ技术进行了优化，并用此法联合检测了１０５例江苏地区健康人群及６８例肺癌患者ＣＹＰ１Ａ１、ＧＳＴＭ１的等位基因型。结果表明：ＡＳＰＣＲ及ＭＤＰＣＲ采用的设立双参照扩增体系，可一次同时检测ＣＹＰ１Ａ１和ＧＳＴＭ１的等位基因型。在肺癌患者组中，ＣＹＰ１Ａ１的突变型Ｖａｌ／Ｖａｌ的频率１２／６８（１７．６％）约为健康组９／１０５（８．５７％）的２０５倍，而ＧＳＴＭ１纯合缺失的频率，肺癌组为３９／６８（５７３％），与健康对照组４２／１０５（４０％）相比，亦有显著增加（Ｐ＜０．０５）。
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| 关 键 词： | 等位基因特异性PCR；多重差别PCR；遗传多态；肺癌 |
IDENTIFICATION OF GENETIC POLYMORPHISM OF CYP1A1 AND GSTM1 IN LUNG CANCER PATIENTS BY USING ALLELESPECIFIC PCR AND MULTIPLEX DIFFERENTIAL PCR |
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| Abstract: | Allelespecific PCR(ASPCR) and multiplex differential PCR (MDPCR) were used to identify the genotypes of CYP1A1 and GSTM1 in 68 lung cancer patients and 105 healthy controls. The results showed that genotypes of CYP1A1 and GSTM1 could be detected simultaneously with ASPCR and MDPCR; The frequency of CYP1A1Val/Val in lung cancer patients group was 12/68(17.6%), about 2.05fold than that in healthy controls 9/105(8.57%), and the frequency of GSTM1 0/0 was 39/68(57.4%), remarkably higher compared with 42/105(40%) in healthy controls ( P <0.05) |
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| Keywords: | allelespecific PCR multiplex differential PCR polymorphism CYP1A1 GSTM1 |
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