伴微乳头结构的肺腺癌恶性潜能机制研究

  目的  探讨伴微乳头成分的肺腺癌(pulmonary adenocarcinoma with a micropapillary pattern, MPPAC)上皮间质转化和增殖调亡特征, 为临床评价其恶性潜能提供理论依据。  方法  根据2011年发表的肺腺癌新分类诊断标准, 以是否伴有微小乳头状结构(micropapillary pattern, MPP)将120例肺腺癌病例分为64例MPP阳性组, 56例MPP阴性组。免疫组织化学法检测Vimentin、E-cadherin、Capase-3、pEGFR的表达。  结果  120例肺腺癌病例中pECFR在女性(P=0.035)、非吸烟(P=0.017)患者中高表达。Vimentin在MPP阳性组的表达(28.1%)高于MPP阴性组(16.1%)并且其多表达于微乳头结构腔内散落的细胞; Caspase-3在MPP阳性组高表达(P=0.001)。  结论  微乳头结构的腔内散落细胞表现间质表型有利于癌细胞浸润转移, 伴乳头结构的肺腺癌有其独特的分子生物学特征。 

Epithelial-mesenchymal Transition Cell Proliferation and Cell Apoptosis in Pulmonary Adenocarcinoma with a Micropapillary Pattern

  Objective  This paper aimed to determine the characteristics of epithelial-mesenchymal transition, cell proliferation, and cell apoptosis in pulmonary adenocarcinoma with a micropapillary pattern(MPPAC).  Methods  Based on the new diagnostic criteria for lung adenocarcinoma classification(published in 2011), 120 cases of lung adenocarcinoma were divided into micropapillary pattern (MPP)-positive(n=64)and MPP-negative(n=54)groups.The expression of vimentin, E-cadherin, caspase-3, and pEGFR in pulmonary adenocarcinoma tissue specimens was determined by immunohistochemical assay.  Results  pEGFR expression in 120 cases of lung adenocarcinoma was higher in female(P=0.035)and nonsmoking(P=0.017)patients.Vimentin expression was higher in the MPP-positive(28.1%)than MPP-negative(16.1%)groups, and mostly located in scattered cells in the MPP cavity.Caspase-3 expression was significantly higher in the MPP-positive group(P=0.001).  Conclusion  Scattered cells in the MPP cavity have a mesenchymal phenotype, which may favor invasion and metastasis, indicating a unique characteristic of MPPAC.