华东地区结核病患者治疗前血清IFN-γ和IL-10水平及其影响因素

 目的  探讨华东地区结核病患者治疗前体内Th1/Th2细胞因子水平及影响因素,为结核病诊疗提供有效信息。方法  采用自行设计的调查问卷,选择华东两省4个研究现场结核病防治定点门诊连续调查1年(2013年4月—2014年3月),调查对象为新诊断登记的活动性结核病患者。ELISA法检测血清中细胞因子IFN-γ和IL-10水平。结果  共计纳入1 289名活动性结核病患者,患者中咳嗽(83.1%)、咳痰(75.5%)症状出现频率最高。调查对象治疗前体内IFN-γ和IL-10因子水平分别为(2.086±1.119)pg/mL和(2.576±1.192)pg/mL,Th1/Th2比值为0.81(CIFN-γ/CIL-10,C代表因子浓度)。咳嗽、咳痰的患者体内IFN-γ水平较低,发热、乏力、夜间盗汗的患者IFN-γ较高(P<0.05)。初治结核病患者体内IFN-γ水平高于复治患者(P<0.05)。饮茶相对不饮茶患者体内IFN-γ水平高(P<0.05)。饮酒相对于不饮酒、规律均衡营养相对于偏食患者体内IL-10水平低(P<0.05)。结论  结核病患者就诊症状值得关注,健康的饮食习惯或可帮助获得较高的免疫水平并纠正结核病患者病程中的Th1/Th2细胞因子失衡。

The serum levels of IFN-γ and IL-10 in tuberculosis patients from East China before anti-tuberculosis treatment and the impact factors

Objective  To describe the serum levels of Th1/Th2 cytokines of tuberculosis (TB) patients from East China before anti-TB treatment and the impact factors in order to provide information for TB diagnosis and treatment.Methods   Four fields of TB appointed hospitals from two provinces in East China were selected.All newly diagnosed and registered active TB patients from Apr.,2013 to Mar.,2014 were investigated by self-designed questionnaire.ELISA was used to test the levels of IFN-γ and IL-10 in the serum.Results   Totally 1 289 active TB patients were enrolled in the study sites,of whom 83.1% and 75.5% were reported having cough and sputum,respectively.The levels of IFN-γ and IL-10 were (2.086±1.119) pg/mL and (2.576±1.192) pg/mL,respectively.Theratio of Th1/Th2 was 0.81 (CIFN-γ/CIL-10,C stands for concentration).The level of IFN-γ was lower in patients with cough and sputum,while it was higher in those with fever,fatigue and night sweats (P<0.05).In addition,the level of IFN-γ in initial treatment patients was higher than that in retreatment cases (P<0.05).It was found that drinking tea could raise the level of IFN-γ (P<0.05),drinking wine and taking a regular balanced diet could reduce the level of IL-10 (P<0.05).Conclusions  The symptoms of TB patients deserved attention.A healthy eating habits can help to achieve a high level of immunity and to correct the imbalance of Th1/Th2 cytokines in the course of disease.