玄柏爽声颗粒HPLC指纹图谱研究

 目的 建立玄柏爽声颗粒HPLC指纹图谱,为其质量控制提供依据。方法 采用Agilent TC C18 (150 mm×4.6 mm, 5 μm) 色谱柱,流动相为甲醇-0.2%乙酸水,0~60 min内进行梯度洗脱;柱温25 ℃,流速1 mL/min,检测波长278 nm。分别对11批样品进行测定,采用“中药指纹图谱计算机辅助相似度评价软件(2004A版)”建立玄柏爽声颗粒HPLC指纹图谱,并对共有峰进行药材归属和指认。结果 建立了玄柏爽声颗粒HPLC指纹图谱,确定了27个共有峰,11批样品的相似度均在0.9以上。各共有峰均能在对应药材中得到归属。用对照品指认了制剂中5个色谱峰,分别为黄柏碱(11号峰)、甘草苷(22号峰)、安格洛苷C(25号峰)、肉桂酸(26号峰)、哈巴俄苷(27号峰)。结论 该方法简便、准确、重现性好、稳定性好,可作为玄柏爽声颗粒的质量评价方法。

HPLC fingerprint of Xuanbo Shuangsheng Granule

Objective To establish the HPLC fingerprint of Xuanbo Shuangsheng Granule for its quality control. Methods The components were separated on an Agilent TC-C18 column (150 mm×4.6 mm, 5 μm) at 25 ℃, with a gradient elution in 0-60 min at the flow rate of 1 mL/min using 0.2% acetic acid aqueous solution and methanol as the mobile phase. The detection wavelength was 278 nm. By detecting 11 batches of Xuanbo Shuangsheng Granule, the HPLC fingerprint was established using “Similarity Evaluation System for Chromatographic Fingerprint of TCMs (Version 2004A)”and the common peaks were analyzed and identified in raw herbal material. Results The HPLC fingerprint of Xuanbo Shuangsheng Granule was obtained with similarity all over 0.9. Totally 27 common peaks were confirmed, and each common peak could be found in raw herbal medicines. Based on the reference substances, 5 common peaks were identified,including phellodendrine (peak 11), liquiritin (peak 22), angoroside C (peak 25), cinnamic acid (peak 26) and harpagoside (peak 27). Conclusions This method is simple, accurate, repeatable and reliable, which could be applied in the quality control of Xuanbo Shuangsheng Granule.