| 分离纯化大鼠肺泡Ⅱ型细胞中水通道蛋白-4的表达 |
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| 引用本文： | 刘琳,李涛平.分离纯化大鼠肺泡Ⅱ型细胞中水通道蛋白-4的表达[J].第一军医大学学报,2005,25(7):784-786. |
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| 作者姓名： | 刘琳 李涛平 |
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| 作者单位： | 南方医科大学南方医院呼吸科,广东广州510515 |
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| 摘 要： | 目的观察经分离纯化的大鼠肺泡Ⅱ型上皮细胞水通道蛋白-4(AQP4)的表达。方法对大鼠肺泡Ⅱ型上皮细胞进行分离、纯化，绎过特殊染色及电镜鉴定，用免疫细胞化学及免疫电镜方法检测水通道蛋白-4存该单细胞上的表达。结果大鼠肺泡Ⅱ型细胞免疫细胞化学水通道蛋白1—4呈强阳性，免疫电镜结果细胞膜上可见高电子密度阳性反应。结论水通道蛋白-4存存于分离的大鼠肺泡Ⅱ型细胞膜上，对呼吸系统水转运可能起到重要作用。
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| 关 键 词： | 水通道蛋白 肺泡Ⅱ型细胞 |
Expression of aquaporin-4 in isolated and purified rat alveolar type II cells] |
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| Lin Liu,Tao-ping Li.Expression of aquaporin-4 in isolated and purified rat alveolar type II cells][J].Journal of First Military Medical University,2005,25(7):784-786. |
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| Authors: | Lin Liu Tao-ping Li |
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| Institution: | Department of Respiratory Medicine, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. liu1306@sohu.com |
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| Abstract: | OBJECTIVE: To explore the expression of aquaporin-4 (AQP4) in isolated and purified rat alveolar type II cells. METHODS: Rat alveolar type II cells were isolated, purified and identified by special staining and electron microscopy. The distribution of AQP4 in alveolar type II cells was observed by immunocytochemistry and immunoelectron microscope with affinity-purified antibodies to human AQP4. RESULTS: Immunocytochemistry showed staining for AQP4 in rat alveolar type II cells, and immunoelectron microscope demonstrated the presence of AQP4 on the membrane of the cells. CONCLUSION: AQP4 is expressed in alveolar type II cells in rats, and may play a role in water transportation in the respiratory system. |
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