| Expression of receptor for advanced glycosylation end products(AGEP)and inhibition of AGEP-induced cytosolic calcium elevationbydiltiazeminculturedrataorticsmoothmusclecels |
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| 引用本文： | 周秋根,刘乃丰,谢陪俐.Expression of receptor for advanced glycosylation end products(AGEP)and inhibition of AGEP-induced cytosolic calcium elevationbydiltiazeminculturedrataorticsmoothmusclecels[J].中国药理学报(英文版),1997(5). |
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| 作者姓名： | 周秋根 刘乃丰 谢陪俐 |
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| 作者单位： | 南京铁道医学院 |
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| 摘 要： | 目的：探讨培养的主动脉平滑肌细胞（ＡＳＭＣ）上是否表达糖基化终产物（ＡＧＥＰ）高亲性受体和地尔硫对ＡＧＥＰ升高胞浆游离钙的抑制．方法：用放射配基结合方法研究ＡＳＭＣ与ＡＧＥＰ的相互作用；用钙离子荧光指示剂Ｆｕｒａ２ＡＭ测定ＡＳＭＣ胞浆游离钙．结果：ＡＳＭＣ上有ＡＧＥＰ高亲和性受体表达，其解离常数为６５３±１５ｎｍｏｌ·Ｌ－１，最大结合容量１５７±００４ｎｍｏｌ／ｇ细胞蛋白；通过该受体介导，平滑肌细胞可内化、代谢ＡＧＥＰ．ＡＧＥＰ使胞浆游离钙呈浓度依赖性升高；地尔硫呈时间、浓度依赖性抑制此效应．结论：大鼠ＡＳＭＣ上有ＡＧＥＰ高亲和性受体表达；地尔硫可抑制ＡＧＥＰ介导的胞浆游离钙升高．
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| 关 键 词： | 放射配基测定 糖基化终产物 钙 地尔硫 血管平滑肌 培养的细胞 糖尿病血管病变 动脉粥样硬化 胸主动脉 |
Expression of receptor for advanced glycosylation end products (AGEP) and inhibition of AGEP induced cytosolic calcium elevation by diltiazem in cultured rat aortic smooth muscle cells |
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| ZHOU Qiu Gen,LIU Nai Feng,XIE Pei Li.Expression of receptor for advanced glycosylation end products (AGEP) and inhibition of AGEP induced cytosolic calcium elevation by diltiazem in cultured rat aortic smooth muscle cells[J].Acta Pharmacologica Sinica,1997(5). |
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| Authors: | ZHOU Qiu Gen LIU Nai Feng XIE Pei Li |
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| Institution: | ZHOU Qiu Gen,LIU Nai Feng,XIE Pei Li 2 |
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| Abstract: | |
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| Keywords: | radioligand assay advanced glycosylation end products calcium diltiazem vascular smooth muscle cultured cells diabetic angiopathies atherosclerosis thoracic aorta |
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