| RP-HPLC法测定五苓散中麦角甾醇和桂皮醛的含量 |
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| 引用本文： | 李彧,姜君,赵春杰.RP-HPLC法测定五苓散中麦角甾醇和桂皮醛的含量[J].海峡药学,2011,23(6):58-60. |
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| 作者姓名： | 李彧 姜君 赵春杰 |
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| 作者单位： | 1. 沈阳药科大学药学院,沈阳,110016;福建中医药大学药学院,福州,350108
2. 沈阳药科大学药学院,沈阳,110016 |
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| 摘 要： | 目的建立五苓散中麦角甾醇和桂皮醛的含量测定方法。方法五苓散样品经甲醇超声提取后,用HPLC法测定。色谱柱为HypersllC18（250mm×4.6mm,5μm）,流动相为甲醇-水梯度洗脱,流速为1mL.min-1,检测波长为283nm,柱温为30℃。结果麦角甾醇在0.0437~1.3098mg.mL-1浓度范围内线性关系良好（r=0.9991）,平均回收率为102.9%,RSD为1.5%（n=6）;桂皮醛在0。0062~0.1872mg.mL-1浓度范围内线性关系良好（r=1.0000）,平均回收率为97.8%,RSD为1.9%（n=6）。结论该方法简便、准确、可靠,可用于五苓散中麦角甾醇和桂皮醛的同时含量测定。
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| 关 键 词： | 五苓散 麦角甾醇 桂皮醛 含量测定 |
Determination of ergosterol and cinnamaldehyde in Wulingsan by HPLC |
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| LI Yu,JIANG Jun,ZHAO Chun-jie.Determination of ergosterol and cinnamaldehyde in Wulingsan by HPLC[J].Strait Pharmaceutical Journal,2011,23(6):58-60. |
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| Authors: | LI Yu JIANG Jun ZHAO Chun-jie |
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| Institution: | 1(1.School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China;2.School of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350108,China) |
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| Abstract: | OBJECTIVE To establish a RP-HPLC method for quantitative determination of ergosterol and cinnamaldehyde in Wulingsan.METHODS Wulingsan was extracted with methanol by ultrasound extraction.The separation was performed on a Hypersil C18 column(4.6mm×250mm,5μm) at 30℃ using a gradient elution consisting of mobile phase A(methanol) and mobile phase B(water).The detection wavelength was 283nm.RESULTS Ergosterol showed a good linear relationship at the range of 0.0437~1.3098mg·mL-1,and cinnamaldehyde was from 0.0062 to 0.1872mg·mL-1.The average recovery of ergosterol was 102.9%(RSD=1.5%,n=6),cinnamaldehyde was 97.8%(RSD=1.9%,n=6).CONCLUSION The developed method is simple,accurate and reliable with good repeatability,and can be used for routine analysis of ergosterol and cinnamaldehyde in Wulingsan. |
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| Keywords: | Wulingsan ergosterol cinnamaldehyde HPLC |
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