| 高效液相色谱法测定伤痛宁膏中盐酸小檗碱的含量 |
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| 引用本文： | 陈捷,庄惠清.高效液相色谱法测定伤痛宁膏中盐酸小檗碱的含量[J].海峡药学,2011,23(6):60-61. |
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| 作者姓名： | 陈捷 庄惠清 |
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| 作者单位： | 1. 万源(福州)药业有限公司,福州,350014
2. 福建福州市药品检验所,福州,350009 |
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| 摘 要： | 目的建立高效液相色谱法测定伤痛宁膏中盐酸小檗碱的含量。方法采用Kromasil C18色谱柱;乙腈∶0.1%磷酸溶液（50∶50）（每100mL加十二烷基磺酸钠0.1g）为流动相;流速：1mL.min-1;检测波长265nm。结果盐酸小檗碱进样量在0.3069-1.5345μg范围内与峰面积呈良好的线性关系（r=0.9999）,平均回收率为99.04%,RSD=0.96%（n=9）;结论该方法简便,快捷,准确度高,可用于伤痛灵膏中小檗碱的含量测定。
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| 关 键 词： | 伤痛灵膏 盐酸小檗碱 高效液相色谱法 |
Determination of Berberine Hydrochloride in Shangtongning plaster by HPLC |
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| CHEN Jie,ZHUANG Hui-qing.Determination of Berberine Hydrochloride in Shangtongning plaster by HPLC[J].Strait Pharmaceutical Journal,2011,23(6):60-61. |
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| Authors: | CHEN Jie ZHUANG Hui-qing |
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| Institution: | 1.Man Yuan(Fuzhou) Medicine CO.,LTD,Fuzhou 350014,China;2.Fuzhou Institute of Drug Control,Fuzhou,350009,China) |
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| Abstract: | OBJECTIVE To establish a HPLC method for determine quantitatively of berberine in Shangtongning plaster.METHODS The column was Kromasil C18;the mobile phase was acetonitrile-0.1% phosphoric acid solution(50∶50,V/V)(100mL per 0.1g of SDS);the flow rate was 1.0mL·min-1;the detective wavelength was 365nm.RESULTS The linear range of Berberine Hydrochloride was 0.3069~1.5345μg(r=0.9999),the The average recovery rate was 99.04%,and RSD was 0.96%;CONCLUSION The method is simple,rapid,accurate,and can be applied to the determination of berberine in Shangtongning plaster. |
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| Keywords: | Shangtongning plaster Berberine Hydrochloride HPLC |
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