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| 丹参酮Ⅱ_A对HeLa宫颈癌细胞凋亡的影响 | |
| 引用本文： | 沈隽,王照艳,张晓,杜利力,翟爱民,邱文生,葛银林. 丹参酮Ⅱ_A对HeLa宫颈癌细胞凋亡的影响[J]. 中国药房, 2007, 18(27): 2102-2104 |
| 作者姓名： | 沈隽 王照艳 张晓 杜利力 翟爱民 邱文生 葛银林 |
| 作者单位： | 1. 青岛市商业职工医院,青岛市,266011 2. 青岛市市立医院,青岛市,266011 3. 青岛大学医学院,青岛市,266021 4. 青岛大学医学院附属医院,青岛市,266003 |
| 摘 要： | 目的:探讨丹参酮ⅡA对HeLa宫颈癌细胞凋亡的影响及其作用机制。方法:应用MTT比色法检测不同浓度丹参酮ⅡA对HeLa宫颈癌细胞的增殖抑制,采用烟酸己可碱33258染色法观察丹参酮ⅡA对HeLa细胞凋亡的影响,并用半定量逆转录聚合酶链反应法检测用药后Bcl-2和bax基因mRNA的表达水平。结果:丹参酮ⅡA对HeLa细胞增殖的抑制作用呈剂量依赖性;它能促使HeLa细胞发生凋亡;用药48h后Bcl-2基因mRNA的表达水平明显降低,而bax基因的表达则无明显变化。结论:丹参酮ⅡA对HeLa细胞的增殖具有显著的抑制及诱导凋亡作用。 |
| 关 键 词： | 丹参酮Ⅱ_A HeLa细胞株 凋亡 |
| 文章编号： | 1001-0408（2007）27-2102-03 |
| 收稿时间： | 2007-02-15 |
| 修稿时间： | 2007-05-08 |
Induction of Apoptosis by Tanshinone Ⅱ A in HeLa Cells |
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| SHEN Jun,WANG Zhaoyan,ZHANG Xiao,DU Lili,ZHAI Aimin,QIU Wensheng,GE Yinlin. Induction of Apoptosis by Tanshinone Ⅱ A in HeLa Cells[J]. China Pharmacy, 2007, 18(27): 2102-2104 | |
| Authors: | SHEN Jun WANG Zhaoyan ZHANG Xiao DU Lili ZHAI Aimin QIU Wensheng GE Yinlin |
| Affiliation: | Oingdao Commercial 5tall-worker Plospital, Qingdao 266011,China;Qingdao Municipal Hospital, Qingdao 266011, China;Medical College of Qingdao University, Qingdao 266021, QIU Wensheng(The Affiliated Hospital of Medical College of Qingdao University, China |
| Abstract: | OBJECTIVE:To discuss the effect of tanshinone Ⅱ A on the apoptosis of HeLa cell line and its mechanism.METHODS:MTT was used to measure the levels of the proliferation of HeLa cells cultured with tanshinone Ⅱ A in different concentrations.The apoptosis was measured by Hoechst33258 staining,and the level of mRNA of Bcl-2 and bax in HeLa cells was tested by semi-fix quantify RT-PCR method.RESULTS:Tanshinone Ⅱ A could inhibit the proliferation of HeLa cells in a dose dependent manner,induce cell apoptosis,and markedly decrease the expression of Bcl-2 on mRNA level after treatment for 48h,while it had no obvious change in bax mRNA level.CONCLUSION:Tanshinone Ⅱ A can inhibit the proliferation of HeLa cells and induce the apoptosis of the cells. |
| Keywords: | Tanshinone Ⅱ_A HeLa cells Apoptosis |
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