维生素D摄入水平对孕鼠骨代谢的影响

目的:研究大鼠孕期维生素D摄入水平对胎鼠的影响。方法:24只怀孕大鼠随机分为Ⅰ、Ⅱ、Ⅲ组,Ⅰ组为正常对照组(每公斤饲料含维生素D 1 500 IU),Ⅱ组为补充维生素D组(每公斤饲料含维生素D 5 000 IU),Ⅲ组为缺维生素D组(饲料中不添加维生素D)。用含有不同维生素D剂量的饲料喂养怀孕大鼠,观察孕鼠血清钙、磷水平的变化及尿钙和尿羟脯氨酸的变化,观察新生鼠的体重、身长、尾长。计算各组孕鼠平均产仔数。结果:与对照组比较,Ⅱ组孕鼠尿钙升高(P<0.05),尿羟脯氨酸无变化,Ⅲ组孕鼠尿钙和羟脯氨酸均升高(P<0.01);各组子鼠骨骼无畸形,发育指标无统计学差异;平均每只大鼠的产仔数目以补充维生素D组最多(平均每只孕鼠产仔11只),缺维生素D组最少(平均每只孕鼠产仔4只)。结论:孕期缺乏维生素D会使孕鼠骨吸收增强,产仔数减少,未发现维生素D缺乏对子代大鼠发育的影响。

Effect of different vitamin D intakes on bone metabolism of pregnant rats

Objective:To investigate the effects of different intakes for Vitamin D on bone metabolism of pregnant rats.Methods:24 pregnant rats were divided into three groups: group A(control group,1500IU/kg),group B(sufficient Vitamin D feed,5000 IU/kg) and group C(lack Vitamin D feed).At the 17th day of pregnant,blood and urine were collected by metabolism experiment.Blood calcium,phosphate,urine calcium and hydroxyproline were tested.Newborns were tested for their weight,body length,tail length and their skeleton were stained.Results:The urine calcium of the pregnant rats in group B improved(P<0.05).The urine calcium and urine hydroxyproline of the pregnant rats in group C increased(P<0.01).The number of newborn were different between groups.The rats in group C produce less babies than other groups.The all newborns were normal in weight,length and skeleton.Conclusion:The inadequate of Vitamin D in feed can make the mother rats skeleton loss and the number of newborn decrease,but no influence is found in their baby's weight,length,skeleton.