绝经后女性骨质疏松与OPG、RANKL和ApoE的相关性研究

目的:通过观察去卵巢小鼠股骨内OPG、RANKL和ApoE的变化来探讨绝经后女性骨质疏松的发病机制。方法:采用双侧卵巢切除术制备骨质疏松的动物模型,1周后雌激素替代组开始腹腔注射苯甲酸雌二醇(EB)。6周后应用免疫组化法观察小鼠股骨内OPG、RANKL、ApoE的变化。结果:去卵巢组股骨内OPG、RANKL和ApoE的表达与对照组相比明显下降,而补充雌激素后OPG、RANKL和ApoE的表达明显上升。结论:绝经后女性骨质疏松与雌激素具有相关性,雌激素可通过增加OPG、RANKL和ApoE的表达抗骨质疏松。

Relationship of OPG, RANKL, ApoE and osteoporosis in post -menopause women

Objective:To discuss the mechanism of osteoporosis in post-menopause women through investigating influence of estrogen on OPG,RANKL and ApoE in ovariectomized mice.Methods:All mice were divided into three groups-control groups,ovariectomized groups and estrogen-complemented groups.One week later,estrogen-complemented mice were infused with estradiol benzoate twice each week.Control groups and ovariectomized groups were infused with the same does of olive oil.Six weeks later,immunohistochemistry and image-analysis were used to test the changes of OPG,RANKL and ApoE in each group.Results:The level of OPG,RANKL and ApoE were increased in estrogen-complemented groups,but decreased in ovariectomized groups.Conclusion:There is close relationship between estrogen and osteoporosis in post-menopause women.Estradiol benzoate improves the level of OPG,RANKL and ApoE to resist osteoporosis.