人细小病毒B19感染与习惯性流产的关系探讨

目的:探讨人细小病毒B 19(Human parvovirus B 19,HPVB19)感染与习惯性流产(Recurrent pregnancy loss,RPL)的关系。方法:建立人细小病毒B 19巢式聚合酶链反应(Nested Polymerase chain reaction,NestedPCR)检测方法,采集习惯性流产孕妇流产组织和正常妊娠人工流产组织,用巢式PCR方法检测流产组织中HPV B 19 DNA。结果:56例RPL孕妇流产组织中HPVB 19 DNA有16例阳性,阳性率28.6%,50例正常妊娠人工流产组织中HPVB19 DNA有2例阳性,阳性率4.0%,2组HPV B19感染率有显著性差异(χ2=12.38,P<0.01)。流产次数与HPV DNA阳性率无显著性关系(χ2=0.03,P>0.05)。结论:RPL孕妇HPV B 19感染率显著高于正常孕妇,HPV B 19感染可能导致习惯性流产的发生。

The relationship between human parvovirus B19 and recurrent pregnancy loss

Objective:To investigate the relationship between recurrent pregnancy loss(RPL) and human parvovirus B19(HPV B19) infection.Methods:56 abortion tissue specimens from the pregnancy women with RPL and 50 abortion tissue specimens from the normal pregnancy woman were collected.The total DNA were separated from abortion tissue specimens,and HPV B 19 DNA was detected by nested polymerase chain reaction(nested PCR).Results:28.6% pregnancy women with RPL were HPV B19 DNA positive and only 4.0% normal pregnancy women were HPV B19DNA positive.There was no relationship between the number of abortion and the positive ratio of HPV B19 DNA.Conclusion:The rate of HPV B19 infection in pregnancy woman with RPL markedly is higher than that of normal pregnancy woman,it suggests that HPV B 19 perhaps is one of factor of RPL.